- Open Access
Estrous cycle influences excitatory amino acid transport and visceral pain sensitivity in the rat: effects of early-life stress
- Rachel D. Moloney†1, 3, 4,
- Jahangir Sajjad†2,
- Tara Foley2,
- Valeria D. Felice1,
- Timothy G. Dinan1, 3,
- John F. Cryan1, 2 and
- Siobhain M. O’Mahony1, 2Email author
© The Author(s). 2016
Received: 12 May 2016
Accepted: 1 July 2016
Published: 14 July 2016
Early-life stress (ELS) is a recognized risk factor for chronic pain disorders, and females appear to be more sensitive to the negative effects of stress. Moreover, estrous cycle-related fluctuations in estrogen levels have been linked with alternating pain sensitivity. Aberrant central circuitry involving both the anterior cingulate cortex (ACC) and the lumbosacral spinal cord has also been implicated in the modulation of visceral pain in clinical and preclinical studies. Here we further investigate changes in visceral pain sensitivity and central glutamatergic systems in rats with respect to estrous cycle and ELS.
We investigated visceral sensitivity in adult female Sprague-Dawley rats, which had undergone maternal separation (MS) in early life or remained non-separated (NS), by performing colorectal distension (CRD). We also assessed excitatory amino acid uptake through excitatory amino acid transporters (EAATs) in the lumbosacral spinal cord and ACC.
NS animals in proestrus and estrus exhibited reduced EAAT uptake and decreased threshold to CRD. Moreover, total pain behaviors were increased in these stages. MS rats exhibited lower pain thresholds and higher total pain behaviors to CRD across all stages of the estrous cycle. Interestingly, cortical EAAT function in MS rats was inhibited in the low estrogen state—an effect completely opposite to that seen in NS rats.
This data confirms that estrous cycle and ELS are significant factors in visceral sensitivity and fluctuations in EAAT function may be a perpetuating factor mediating central sensitization.
Chronic pain syndromes such as irritable bowel syndrome (IBS), fibromyalgia, migraine, and interstitial cystitis display a striking female preponderance with females presenting at the clinic up to ten times more often than their male counterparts [1, 2]. Moreover, females report more intense pain, of longer duration, and more frequently. With these high prevalence rates, a growing body of evidence suggests that gonadal hormones play a significant role in pain processing . In line with this, recent evidence suggests the possible interaction of gonadal hormones with the brain-gut axis and downstream pain processing [4–6]. Indeed, gonadal hormone binding sites are widely distributed in areas of the central nervous system (CNS) involved in pain processing. Sex steroids also have receptors located throughout the entire intestine, with both estrogen and progesterone exhibiting direct effects on visceral organs . Furthermore, estrogen has been implicated in the possible modulation of visceral pain perception [5, 8]. However, both clinical studies and animal models have revealed conflicting evidence on the role of the female hormonal cycle on pain perception [9, 10]. Numerous studies in the rat have shown a decreased threshold and greater sensitivity in the proestrus phase; however, others have reported no difference between estrous stages [5, 11–13]. These discrepancies may be due in part to strain differences and the inaccuracy of estrous cycle determination.
Estrogen receptors have also been shown to interact with other key neurotransmitter systems such as the glutamatergic system, which itself is critical to pain processing mechanisms . In particular, the process of central sensitization implicates a critical role of excessive glutamatergic signaling leading to the development of plasticity, thus maintaining the chronic pain state [15, 16]. The glial excitatory amino acid transporters (EAATs), in particular, EAAT 1 and EAAT 2 are crucial in the maintenance of homeostasis within the glutamatergic synapse; however, their expression has been shown to be altered in chronic pain models [17, 18]. Intrathecal administration of EAAT blockers in naïve animals results in spontaneous somatic pain, as well as hyperalgesia and allodynia, implying that a continuous spinal glutamate uptake has a key basal anti-nociceptive action [19–21]. Moreover, EAAT 2 has also been shown to play a vital role in numerous models of visceral pain  with increased expression showing anti-nociceptive effects . Moreover, pharmacological activation of these transporters has also demonstrated therapeutic potential .
Many chronic pain disorders are also included in categories of stress-induced disorders as a significant proportion of patients attribute the onset and exacerbation of their symptoms to an early-life stressful period or chronic stress throughout life. Indeed, it is now becoming more evident that females appear more sensitive to the negative effects of stress . Early-life stress (ELS) during childhood is related to increased risk to develop depression, anxiety, and chronic pain in adulthood . Maternal separation in the early days of life in animals is a widely used model of ELS and has been used to elucidate the underlying mechanisms of depression as well as chronic pain disorders [26–28]. The model is based on the evidence that adverse environmental alterations during early life can cause long-lasting effects into adulthood e.g., increased visceral pain sensitivity . In the present study, we aimed to assess whether maternal separation and the estrous cycle alter visceral sensitivity and if changes in phenotype were associated with changes in excitatory amino acid transport via glial uptake in the lumbosacral spinal cord and the anterior cingulate cortex (ACC), two critical regions within the CNS known to play a role in visceral pain processing [26, 29].
Adult male and female Sprague-Dawley rats (250–300 g) (Harlan, UK) were used as breeding partners to generate offspring in this study. Upon arrival, animals were housed according to sex, four to five animals per cage, in plastic cages and were maintained in a temperature-controlled room (20 ± 1 °C) with a 12-h light/dark cycle (7:00 am to 7:00 pm). The animals were allowed 1 week to acclimatize to the animal facility in University College Cork after arrival. Breeding pairs were housed together until confirmation of pregnancy. Females were then group housed throughout gestation until gestation day 19 after which time they were housed singly and allowed to give birth. Two cohorts of animals were used in the current study. (1) The first cohort was used for behavioral analysis of visceral sensitivity. (2) The second cohort was used for naïve sample collection to assess EAAT function. Group sizes were nine to ten animals and was based on previous experiments. All experiments were conducted in accordance with the European Directive 2010/63/EU and approved by the Animal Experimentation Ethics Committee of University College of Cork.
Maternal separation was performed from postnatal day 2 (PND 2) to PND 12 inclusive as previously described [28, 30]. Briefly, litters were randomly assigned to either the non-separated (NS) group or the maternally separated (MS) group. NS animals were left undisturbed except for routine husbandry practices. MS pups were separated daily from their mothers in a separate room and placed in a clean cage with fresh bedding, placed on top of heated pads (30–33 °C) for 3 h from 9:00 am until 12:00 pm after which time animals were placed back in their mother’s home cage. Following the separation period (PND2–PND12), animals were left undisturbed except for weekly cage cleaning. Offspring were weaned and sexed at PND 21. Animals were allowed to mature to adulthood (8 weeks), and all female animals were used for the remainder of the study.
Females were vaginally lavaged daily with saline for at least two consecutive estrous cycles, and cells were immediately viewed under a microscope prior to behavioral assessment. Those rats that were regularly cycling were used in this study. The stage of estrous cycle was determined as previously described . Since metestrus only lasts for a short period (5–6 h) and the plasma estrogen concentration in metestrus do not differ from that in diestrus, data from these two groups of rats were pooled. For cohort 1, the behavioral study, animals were lavaged immediately before balloon insertion into the colorectum, to assess estrous stage during visceral pain measurement. For cohort 2, tissue collection only, the animals underwent vaginal smears for cycle phase estimation immediately before decapitated for tissue collection. Towards the end of each study, there were occasions where the lavaged rats were not in the correct phase (we aimed for nine to ten per group) and then we waited a day or two until we lavaged again to check the stage and if it fit into the groups that required more numbers.
Colorectal distension (CRD) was performed as previously described [31, 32]. Briefly, animals were fasted overnight and anesthetized with isoflurane (3–5 % in oxygen) followed by insertion of a 6-cm latex balloon into the colorectal cavity, 1 cm from the anus. The animals were allowed to recover for 10 min before CRD commenced (9:00 am to 12:00 pm) in unrestrained freely moving animals. The paradigm used was an ascending phasic distension from 0 to 80 mmHg over 8 min. The parameters of interest were (1) the threshold pressure (mmHg) that evokes the first visually identifiable visceral pain behavior and (2) the total number of pain behaviors over the distension period. Postures defined as visceral pain behaviors were abdominal retractions and/or abdominal withdrawal reflex. The experimental groups were randomized, and behavioral testing was performed by an experimenter blinded to treatment groups to eliminate any bias. All animals underwent CRD only once.
Sample preparation for aspartate transport assay
The animals were euthanized by decapitation immediately after vaginal smearing. Their spinal cords were removed by hydraulic pressure into Hank’s balanced salt solution (HBSS)-filled Petri dishes, and 0.4-mm thick slices were obtained from lumbosacral spinal cord using a McIIwain tissue chopper. Similarly, brain tissue was removed from the skull and sectioned using a vibratome to obtain ACC sections. These slices were separated by fine dissection under a microscope and transferred to 24-well culture plates filled with both sodium containing HBSS (labeled as Na+) and sodium-free HBSS (labeled as Na−) separately. Na+ plate was maintained at 35 °C and Na− on the ice. The slices from Na+ plate were washed once with l mL of 35 °C HBSS and Na− plate with l mL of 4 °C sodium-free HBSS to assess sodium-dependent and independent uptake, respectively.
Aspartate transport assay
Since EAATs show high affinity to both glutamate and aspartate, we used aspartate. This protocol has been previously described in brain slices ; we optimized this technique to be used on spinal cord slices for the first time. Aspartic acid, d-[2,3-3H] (specific activity 12.9 Ci/mmol), was purchased from PerkinElmer, USA. RIPA buffer and Pierce BCA protein assay kit were purchased from Fisher Scientific Ireland. All other reagents were purchased from Sigma-Aldrich. HBSS was prepared containing (in mM): 137 NaCl; 0.63 Na2HPO4; 4.17 NaHCO3; 5.36 KCl; 0.44 KH2PO4; 1.26 CaCl2; 0.41 MgSO4; 0.49 MgCl2 and 1.11 glucose, in pH 7.2. In sodium-free HBSS, NaCl was replaced by 137 mM N-methyl-d-glucamine.
Spinal cord and ACC slices were pre-incubated at 35 °C and ice in Na+ and Na− HBSS, respectively, for 30 min. Then 0.66 μCi/ml aspartic acid, d-[2,3-3H], and 100 μM (final concentration) in 20 μl cold d-aspartate was added. After 3 and 7 min of incubation of spinal cord and ACC, respectively, the slices were washed twice with 1 ml of corresponding ice-cold HBSS. Tissue was transferred into 1.5-ml tubes containing RIPA buffer and was mechanically dissociated with pestles. This mixture was homogenized for 15 min at 4 °C and the residue was removed. Radioactivity was measured in terms of counts per minute (CPM) using a liquid scintillation counter and results for Na− samples were subtracted from those of Na+ samples to achieve sodium-dependent uptake-hallmark of EAAT 1 and 2 function. Uptake procedure was performed in triplicate. Protein was measured using Peirce BCA protein assay kit. Final scintillation result for each slice was divided by respective protein value to achieve aspartate uptake in terms of CPM/mg-indicative of spinal and cortical EAAT’s function.
All data was normally distributed according to Gaussian distribution analysis. Data are expressed as mean ± SEM. Two-way analysis of variance (ANOVA) and Tukey post hoc test were used in all cases. p < 0.05 were considered statistically significant. The sample size (n = 9/10) was based on previous studies showing that it was sufficient to observe statistically significant results.
Early-life stress and estrous cycle-dependent variations in visceral sensitivity
A two-way ANOVA analysis of total pain behaviors also revealed a significant effect of stress (F (1, 50) = 8.657, p < 0.01) and a significant effect of estrous cycle (F (2, 50) = 3.571, p < 0.05) but no interaction effect of estrous cycle × stress (F (2, 50) = 1.305, p > 0.05, Fig. 1b, n = 9/10 per group). Overall, MS rats showed a higher number of pain behaviors, with post hoc analysis revealing significantly increased total pain behaviors in the NS animals in the proestrus (p < 0.01) phase compared to NS animals in the metestrus/diestrus phase. There were no between-group differences observed in MS animals.
Early-life stress and estrous cycle-dependent variations in central EAAT activity
Lumbosacral spinal cord
Anterior cingulate cortex
Here we show that ELS and the estrous cycle play significant roles in visceral sensation. MS animals displaying similar visceral pain responses at all stages of the cycle while in NS controls, fluctuations in visceral sensitivity occurred across the cycle. We noted an early-life stress-induced increase in visceral sensitivity in the combined metestrus and diestrus phases but not in the proestrus and estrus phases.
Furthermore, excitatory amino acid transport, both within the lumbosacral spinal cord and the ACC, were also altered in response to stress and estrous cycle, thus indicating that aberrant excitatory transport may in part lead to fluctuations in visceral sensitivity.
Functional gastrointestinal disorders, characterized by visceral pain, have been more commonly reported in females, in particular, premenopausal women, with increasing focus on the effect of hormonal cycles on pain processing . Female IBS patients report increased visceral pain during menses suggesting enhanced visceral sensitivity during the perimenstrual period . The hormonal cycle in the rodent is much shorter than that in humans with the cycle completed in just 4–5 days. Progressing through the stages may occur over the course of just a few hours, making it difficult to investigate the role of the estrous cycle in nociceptive assays due to the inconsistent methodology. For this reason, many studies disregard the cycle [35–37]; however, it is increasingly more appreciated that this is a significant confound and makes the interpretation of data difficult. Indeed, the estrous stage has previously been shown to alter the responses in somatic nociceptive assays, in particular, the tail flick test [10, 38, 39]. This current study found significant changes in visceral sensitivity across the stages of estrous cycle in controls, with heightened visceral pain response in the proestrus and estrous phases. This is in agreement with previous reports [12, 13]; however, it is pertinent to note that other studies have shown findings on the contrary, with evidence showing no changes in visceral pain throughout the cycle . These discrepancies seen in animal models may be due in part not only to species and strain differences but also to the impact of stress. Although not widely acknowledged in the literature, the methodology used to assess estrous stage, i.e., vaginal lavage, can be stressful to animals and may result in changes in the cycle. Thus, it is important to acclimate animals to the vaginal smearing process itself as was performed in this study.
The most potent and prevalent endogenous estrogen, estradiol, is at its highest level during the proestrus phase, which also coincides with the highest level of visceral pain sensitivity, highlighting the potential role of the gonadal hormones in visceral pain. Moreover, its contribution to visceral hypersensitivity has been investigated in animal models through ovariectomy and hormone replacement studies [5, 6]. These studies reported reduced visceral sensitivity in ovariectomized females compared with intact females. Estrogen replacement was reported to increase visceral sensitivity back to levels of that seen in the intact females . Furthermore, the implantation of micropellets containing estradiol and progesterone into the amygdala increased visceral pain behaviors in ovariectomized rats, with no differences seen when placed in neighboring brain regions . Interestingly, no changes were observed in somatic pain thresholds. These findings suggest that female sex steroids do indeed play a significant role in visceral nociception.
Stress and altered hypothalamic-pituitary-adrenal (HPA) axis function is a significant risk factor for the development of visceral pain. Thus, it is interesting to note that hormonal fluctuations have also been shown in stress responsivity and altered neurobiology of the HPA axis. Females have been found to have higher corticotropin-releasing hormone, adrenocorticotropic hormone, and corticosterone levels during proestrus, the phase of the cycle in which estradiol levels are higher than during other phases of the estrous cycle [40–42]. Furthermore, plasma adrenocorticotrophin hormone and corticosterone levels in response to stress are higher during proestrus than during other phases of the cycle [43–45]. Moreover, neurotransmitter systems implicated in the control of HPA function [46, 47] show variations related to the estrous cycle and are sensitive to gonadal steroid levels [48–50]. Indeed, here we specifically focus on the glutamatergic system and the role of glial glutamate transport in visceral nociception.
Here we show that the activity of glutamate transporters in the lumbosacral spinal cord and ACC are significantly decreased during the phases of the estrous cycle when visceral sensitivity is higher. The EAATs are responsible for removing excess glutamate from the synaptic cleft thereby reducing the availability of glutamate and preventing central sensitization. Studies from our own group have shown expression of these transporters is altered in animal models of heightened sensitivity to colorectal distension including the maternal separation model  as well the CBA/J strain of mouse .
Water avoidance stress have also been shown to alter expression levels of these transporters in the spinal cord which was positively correlated with pain behaviors [22, 51]. Taken together, it is plausible that glutamate uptake enhancers may prove to be a novel therapeutic strategy for the treatment of visceral hypersensitivity . Indeed, recent studies show down-regulation of spinal EAAT transporters in various models of chronic pain [52–54]. Moreover, the importance of glial glutamate uptake is further highlighted by reports where EAAT overexpression reduces pain sensitivity [23, 51, 55]. Previous studies have shown higher glutamate concentration in ACC in animals exposed to ELS .
Overall, in MS rats, stress increased visceral sensitivity in metestrus/diestrus phases but had no effect in proestrus and estrus compared to NS rats. The reason for this is not clear, but it is interesting to note that glutamate uptake in the spinal cord paralleled this. However, uptake in the ACC was opposite to the that of the NS controls. This may be compensatory as uptake was higher in the proestrus and estrus in the MS animals, which may indicate that higher activity of these transporters is necessary to maintain behaviors at the same level. Moreover, it appears that visceral sensitivity in MS rats is linked to changes in EAAT function in the ACC, while both the spinal and ACC EAAT function was related to pain behaviors in the NS group. Both ACC and spinal cord EAAT function are reduced in the higher estrogen state i.e., proestrus and ensuing estrus, paralleling that of the pain behaviors. Other studies have indicated that estrogen can affect the expression of the EAATs as well as l-glutamate uptake activity in cultured midbrain astrocytes . Estrogen exerts if effects through either membrane bound or nuclear receptors and stimulation of PI3-kinase coupled to nitric oxide production may be involved in the inhibitory regulation of glutamate transporter activity by estrogen. The presence of nuclear estrogen receptors in astrocytes has been demonstrated in vitro and in vivo . Moreover, it should be appreciated that the expression of estrogen receptors can change  and hence influence the level of impact of estrogen changes also.
Our data further supports an important role of glutamate transport at both the level of the spinal cord and higher brain centers in the pathophysiology of visceral pain.
The findings presented here highlight the important contribution of female sex hormones to the control, processing, and manifestation of visceral pain. Indeed, our studies suggest a complex link between steroid signaling, stress, and glutamatergic neurotransmission. Taken together, these findings have added to the accumulating literature implicating the role of sex hormones and glutamate in visceral pain.
ACC, anterior cingulate cortex; ANOVA, analysis of variance; CRD, colorectal distension; EAAT, excitatory amino acid transporter; ELS, early-life stress; HBSS, Hanks balanced salt solution.; HPA, hypothalamic-pituitary-adrenal axis; IBS, irritable bowel syndrome; MS, maternal separation; NS, non-separated; PND, postnatal day
We wish to thank Prof. David Finn for advising on the aspartate assay and the Department of Biochemistry, UCC for allowing access to equipment.
The Alimentary Pharmabiotic Centre is a research center funded by Science Foundation Ireland (SFI), through the Irish Government’s National Development Plan. Timothy G. Dinan and John F. Cryan are supported by SFI (grant no is 07/CE/B1368 and 12/RC/2273). Timothy G. Dinan and John F. Cryan are also supported by the Irish Health Research Board, Health Research Awards (HRA_POR/2011/23) and (HRA_POR/2012/32).
Availability of data and material
RDM, JS, VDF, and TF performed the research, analyzed the data, and wrote the paper; JFC, TGD, and SMOM conceived of the study, participated in its design and coordination, and helped to draft the manuscript. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
Consent for publication
We agree for consent to publication.
Ethics approval and consent to participate
All experiments were conducted in accordance with the European Directive 2010/63/EU and approved by Animal Experimentation Ethics Committee of University College of Cork.
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- Mogil JS. Sex differences in pain and pain inhibition: multiple explanations of a controversial phenomenon. Nat Rev Neurosci. 2012;13:859–66.View ArticlePubMedGoogle Scholar
- Unruh AM. Gender variations in clinical pain experience. Pain. 1996;65:123–67.View ArticlePubMedGoogle Scholar
- Iacovides S, Avidon I, Baker FC. Does pain vary across the menstrual cycle? A review. Eur J Pain. 2015;19:1389–405.View ArticlePubMedGoogle Scholar
- Heitkemper MM, Jarrett M. Gender differences and hormonal modulation in visceral pain. Curr Pain Headache Rep. 2001;5:35–43.View ArticlePubMedGoogle Scholar
- Ji Y, Murphy AZ, Traub RJ. Estrogen modulates the visceromotor reflex and responses of spinal dorsal horn neurons to colorectal stimulation in the rat. J Neurosci. 2003;23:3908–15.PubMedGoogle Scholar
- Myers B, Schulkin J, Greenwood-Van Meerveld B. Sex steroids localized to the amygdala increase pain responses to visceral stimulation in rats. J Pain. 2011;12:486–94.View ArticlePubMedGoogle Scholar
- Arendt-Nielsen L, Bajaj P, Drewes AM. Visceral pain: gender differences in response to experimental and clinical pain. Eur J Pain. 2004;8:465–72.View ArticlePubMedGoogle Scholar
- Chaloner A, Greenwood-Van Meerveld B. Sexually dimorphic effects of unpredictable early life adversity on visceral pain behavior in a rodent model. J Pain. 2013;14:270–80.View ArticlePubMedGoogle Scholar
- Heitkemper MM, Chang L. Do fluctuations in ovarian hormones affect gastrointestinal symptoms in women with irritable bowel syndrome? Gend Med. 2009;6 Suppl 2:152–67.View ArticlePubMedPubMed CentralGoogle Scholar
- Fillingim RB, Ness TJ. Sex-related hormonal influences on pain and analgesic responses. Neurosci Biobehav Rev. 2000;24:485–501.View ArticlePubMedGoogle Scholar
- Ji Y, Murphy AZ, Traub RJ. Sex differences in morphine-induced analgesia of visceral pain are supraspinally and peripherally mediated. Am J Physiol Regul Integr Comp Physiol. 2006;291:R307–14.View ArticlePubMedGoogle Scholar
- Sapsed-Byrne S, Ma D, Ridout D, Holdcroft A. Estrous cycle phase variations in visceromotor and cardiovascular responses to colonic distension in the anesthetized rat. Brain Res. 1996;742:10–6.View ArticlePubMedGoogle Scholar
- Ji Y, Tang B, Traub RJ. The visceromotor response to colorectal distention fluctuates with the estrous cycle in rats. Neuroscience. 2008;154:1562–7.View ArticlePubMedPubMed CentralGoogle Scholar
- Zhang Y, Xiao X, Zhang XM, Zhao ZQ, Zhang YQ. Estrogen facilitates spinal cord synaptic transmission via membrane-bound estrogen receptors: implications for pain hypersensitivity. J Biol Chem. 2012;287:33268–81.View ArticlePubMedPubMed CentralGoogle Scholar
- David-Pereira A, Puga S, Goncalves S, Amorim D, Silva C, Pertovaara A, Almeida A, Pinto-Ribeiro F. Metabotropic glutamate 5 receptor in the infralimbic cortex contributes to descending pain facilitation in healthy and arthritic animals. Neuroscience. 2016;312:108–19.Google Scholar
- Falnikar A, Hala TJ, Poulsen DJ, Lepore AC. GLT1 overexpression reverses established neuropathic pain-related behavior and attenuates chronic dorsal horn neuron activation following cervical spinal cord injury. Glia. 2016;64(3):396–406.Google Scholar
- Gosselin RD, O'Connor RM, Tramullas M, Julio-Pieper M, Dinan TG, Cryan JF. Riluzole normalizes early-life stress-induced visceral hypersensitivity in rats: role of spinal glutamate reuptake mechanisms. Gastroenterology. 2010;138:2418–25.View ArticlePubMedGoogle Scholar
- Moloney RD, Dinan TG, Cryan JF. Strain-dependent variations in visceral sensitivity: relationship to stress, anxiety and spinal glutamate transporter expression. Genes Brain Behav. 2015;14:319–29.View ArticlePubMedGoogle Scholar
- Aanonsen LM, Wilcox GL. Nociceptive action of excitatory amino acids in the mouse: effects of spinally administered opioids, phencyclidine and sigma agonists. J Pharmacol Exp Ther. 1987;243:9–19.PubMedGoogle Scholar
- Brambilla A, Prudentino A, Grippa N, Borsini F. Pharmacological characterization of AMPA-induced biting behaviour in mice. Eur J Pharmacol. 1996;305:115–7.View ArticlePubMedGoogle Scholar
- Kontinen VK, Meert TF. Vocalization responses after intrathecal administration of ionotropic glutamate receptor agonists in rats. Anesth Analg. 2002;95:997–1001. table of contents.PubMedGoogle Scholar
- Bradesi S, Golovatscka V, Ennes HS, McRoberts JA, Karagiannides I, Bakirtzi K, Pothoulakis C, Mayer EA. Role of astrocytes and altered regulation of spinal glutamatergic neurotransmission in stress-induced visceral hyperalgesia in rats. Am J Physiol Gastrointest Liver Physiol. 2011;301:G580–9.View ArticlePubMedPubMed CentralGoogle Scholar
- Lin Y, Tian G, Roman K, Handy C, Travers JB, Lin CL, Stephens Jr RL. Increased glial glutamate transporter EAAT2 expression reduces visceral nociceptive response in mice. Am J Physiol Gastrointest Liver Physiol. 2009;296:G129–34.View ArticlePubMedGoogle Scholar
- Bangasser DA, Reyes BA, Piel D, Garachh V, Zhang XY, Plona ZM, Van Bockstaele EJ, Beck SG, Valentino RJ. Increased vulnerability of the brain norepinephrine system of females to corticotropin-releasing factor overexpression. Mol Psychiatry. 2013;18:166–73.View ArticlePubMedGoogle Scholar
- Raphael KG, Widom CS. Post-traumatic stress disorder moderates the relation between documented childhood victimization and pain 30 years later. Pain. 2011;152:163–9.View ArticlePubMedGoogle Scholar
- Moloney RD, Stilling RM, Dinan TG, Cryan JF. Early-life stress-induced visceral hypersensitivity and anxiety behavior is reversed by histone deacetylase inhibition. Neurogastroenterol Motil. 2015;27(12):1831–6.Google Scholar
- O'Mahony SM, Hyland NP, Dinan TG, Cryan JF. Maternal separation as a model of brain-gut axis dysfunction. Psychopharmacology (Berl). 2011;214:71–88.View ArticleGoogle Scholar
- O'Mahony SM, Marchesi JR, Scully P, Codling C, Ceolho AM, Quigley EM, Cryan JF, Dinan TG. Early life stress alters behavior, immunity, and microbiota in rats: implications for irritable bowel syndrome and psychiatric illnesses. Biol Psychiatry. 2009;65:263–7.View ArticlePubMedGoogle Scholar
- Lieberman MD, Eisenberger NI. The dorsal anterior cingulate cortex is selective for pain: results from large-scale reverse inference. Proc Natl Acad Sci U S A. 2015;112(49):15250–5.Google Scholar
- Neumann ID, Wigger A, Kromer S, Frank E, Landgraf R, Bosch OJ. Differential effects of periodic maternal separation on adult stress coping in a rat model of extremes in trait anxiety. Neuroscience. 2005;132:867–77.View ArticlePubMedGoogle Scholar
- O'Mahony SM, Bulmer DC, Coelho AM, Fitzgerald P, Bongiovanni C, Lee K, Winchester W, Dinan TG, Cryan JF. 5-HT(2B) receptors modulate visceral hypersensitivity in a stress-sensitive animal model of brain-gut axis dysfunction. Neurogastroenterol Motil. 2010;22:573–8. e124.View ArticlePubMedGoogle Scholar
- O'Mahony SM, Tramullas M, Fitzgerald P, Cryan JF. Rodent models of colorectal distension. Curr Protoc Neurosci. 2012;Chapter 9:Unit 9 40.PubMedGoogle Scholar
- Thomazi AP, Godinho GF, Rodrigues JM, Schwalm FD, Frizzo ME, Moriguchi E, Souza DO, Wofchuk ST. Ontogenetic profile of glutamate uptake in brain structures slices from rats: sensitivity to guanosine. Mech Ageing Dev. 2004;125:475–81.View ArticlePubMedGoogle Scholar
- Mayer EA, Berman S, Chang L, Naliboff BD. Sex-based differences in gastrointestinal pain. Eur J Pain. 2004;8:451–63.View ArticlePubMedGoogle Scholar
- Arvidsson S, Larsson M, Larsson H, Lindstrom E, Martinez V. Assessment of visceral pain-related pseudo-affective responses to colorectal distension in mice by intracolonic manometric recordings. J Pain. 2006;7:108–18.View ArticlePubMedGoogle Scholar
- Tammpere A, Brusberg M, Axenborg J, Hirsch I, Larsson H, Lindstrom E. Evaluation of pseudo-affective responses to noxious colorectal distension in rats by manometric recordings. Pain. 2005;116:220–6.View ArticlePubMedGoogle Scholar
- Kamp EH, Jones 3rd RC, Tillman SR, Gebhart GF. Quantitative assessment and characterization of visceral nociception and hyperalgesia in mice. Am J Physiol Gastrointest Liver Physiol. 2003;284:G434–44.View ArticlePubMedGoogle Scholar
- Martinez-Gomez M, Cruz Y, Salas M, Hudson R, Pacheco P. Assessing pain threshold in the rat: changes with estrus and time of day. Physiol Behav. 1994;55:651–7.View ArticlePubMedGoogle Scholar
- Kayser V, Berkley KJ, Keita H, Gautron M, Guilbaud G. Estrous and sex variations in vocalization thresholds to hindpaw and tail pressure stimulation in the rat. Brain Res. 1996;742:352–4.View ArticlePubMedGoogle Scholar
- Atkinson HC, Waddell BJ. Circadian variation in basal plasma corticosterone and adrenocorticotropin in the rat: sexual dimorphism and changes across the estrous cycle. Endocrinology. 1997;138:3842–8.PubMedGoogle Scholar
- Bohler Jr HC, Zoeller RT, King JC, Rubin BS, Weber R, Merriam GR. Corticotropin releasing hormone mRNA is elevated on the afternoon of proestrus in the parvocellular paraventricular nuclei of the female rat. Brain Res Mol Brain Res. 1990;8:259–62.View ArticlePubMedGoogle Scholar
- Buckingham JC, Dohler KD, Wilson CA. Activity of the pituitary-adrenocortical system and thyroid gland during the oestrous cycle of the rat. J Endocrinol. 1978;78:359–66.View ArticlePubMedGoogle Scholar
- Carey RJ, Pinheiro-Carrera M, Dai H, Tomaz C, Huston JP. L-DOPA and psychosis: evidence for L-DOPA-induced increases in prefrontal cortex dopamine and in serum corticosterone. Biol Psychiatry. 1995;38:669–76.View ArticlePubMedGoogle Scholar
- Pollard I, White BM, Bassett JR, Cairncross KD. Plasma glucocorticoid elevation and desynchronization of the estrous cycle following unpredictable stress in the rat. Behav Biol. 1975;14:103–8.View ArticlePubMedGoogle Scholar
- Viau V, Meaney MJ. Variations in the hypothalamic-pituitary-adrenal response to stress during the estrous cycle in the rat. Endocrinology. 1991;129:2503–11.View ArticlePubMedGoogle Scholar
- Kaneko M, Hiroshige T. Site of fast, rate-sensitive feedback inhibition of adrenocorticotropin secretion during stress. Am J Physiol. 1978;234:R46–51.PubMedGoogle Scholar
- Plotsky PM, Cunningham Jr ET, Widmaier EP. Catecholaminergic modulation of corticotropin-releasing factor and adrenocorticotropin secretion. Endocr Rev. 1989;10:437–58.View ArticlePubMedGoogle Scholar
- Biegon A, McEwen BS. Modulation by estradiol of serotonin receptors in brain. J Neurosci. 1982;2:199–205.PubMedGoogle Scholar
- Rance N, Wise PM, Barraclough CA. Negative feedback effects of progesterone correlated with changes in hypothalamic norepinephrine and dopamine turnover rates, median eminence luteinizing hormone-releasing hormone, and peripheral plasma gonadotropins. Endocrinology. 1981;108:2194–9.View ArticlePubMedGoogle Scholar
- Rance N, Wise PM, Selmanoff MK, Barraclough CA. Catecholamine turnover rates in discrete hypothalamic areas and associated changes in median eminence luteinizing hormone-releasing hormone and serum gonadotropins on proestrus and diestrous day 1. Endocrinology. 1981;108:1795–802.View ArticlePubMedGoogle Scholar
- Lin Y, Roman K, Foust KD, Kaspar BK, Bailey MT, Stephens RL. Glutamate transporter GLT-1 upregulation attenuates visceral nociception and hyperalgesia via spinal mechanisms not related to anti-inflammatory or probiotic effects. Pain Res Treat. 2011;2011:507029.PubMedPubMed CentralGoogle Scholar
- Sung B, Lim G, Mao J. Altered expression and uptake activity of spinal glutamate transporters after nerve injury contribute to the pathogenesis of neuropathic pain in rats. J Neurosci. 2003;23:2899–910.PubMedGoogle Scholar
- Weng HR, Aravindan N, Cata JP, Chen JH, Shaw AD, Dougherty PM. Spinal glial glutamate transporters downregulate in rats with taxol-induced hyperalgesia. Neurosci Lett. 2005;386:18–22.View ArticlePubMedGoogle Scholar
- Mao J, Sung B, Ji RR, Lim G. Chronic morphine induces downregulation of spinal glutamate transporters: implications in morphine tolerance and abnormal pain sensitivity. J Neurosci. 2002;22:8312–23.PubMedGoogle Scholar
- Yang M, Roman K, Chen DF, Wang ZG, Lin Y, Stephens Jr RL. GLT-1 overexpression attenuates bladder nociception and local/cross-organ sensitization of bladder nociception. Am J Physiol Renal Physiol. 2011;300:F1353–9.View ArticlePubMedPubMed CentralGoogle Scholar
- Mathew SJ, Shungu DC, Mao X, Smith EL, Perera GM, Kegeles LS, Perera T, Lisanby SH, Rosenblum LA, Gorman JM, Coplan JD. A magnetic resonance spectroscopic imaging study of adult nonhuman primates exposed to early-life stressors. Biol Psychiatry. 2003;54:727–35.View ArticlePubMedGoogle Scholar
- Pawlak J, Brito V, Kuppers E, Beyer C. Regulation of glutamate transporter GLAST and GLT-1 expression in astrocytes by estrogen. Brain Res Mol Brain Res. 2005;138:1–7.View ArticlePubMedGoogle Scholar
- Garcia-Ovejero D, Veiga S, Garcia-Segura LM, Doncarlos LL. Glial expression of estrogen and androgen receptors after rat brain injury. J Comp Neurol. 2002;450:256–71.View ArticlePubMedGoogle Scholar