Effects of blocking developmental cell death on sexually dimorphic calbindin cell groups in the preoptic area and bed nucleus of the stria terminalis
© Gilmore et al; licensee BioMed Central Ltd. 2012
Received: 22 December 2011
Accepted: 15 February 2012
Published: 15 February 2012
Calbindin-D28 has been used as a marker for the sexually dimorphic nucleus of the preoptic area (SDN-POA). Males have a distinct cluster of calbindin-immunoreactive (ir) cells in the medial preoptic area (CALB-SDN) that is reduced or absent in females. However, it is not clear whether the sex difference is due to the absolute number of calbindin-ir cells or to cell position (that is, spread), and the cellular mechanisms underlying the sex difference are not known. We examined the number of cells in the CALB-SDN and surrounding regions of C57Bl/6 mice and used mice lacking the pro-death gene, Bax, to test the hypothesis that observed sex differences are due to cell death.
Experiment 1 compared the number of cells in the CALB-SDN and surrounding regions in adult males, females, and females injected with estradiol benzoate on the day of birth. In experiment 2, cell number in the CALB-SDN and adjacent regions were compared in wild-type and Bax knockout mice of both sexes. In addition, calbindin-ir cells were quantified within the principal nucleus of the bed nucleus of the stria terminalis (BNSTp), a nearby region that is larger in males due to Bax-dependent cell death.
Males had more cells in the CALB-SDN as well as in surrounding regions than did females, and estradiol treatment of females at birth masculinized both measures. Bax deletion had no effect on cell number in the CALB-SDN or surrounding regions but increased calbindin-ir cell number in the BNSTp.
The sex difference in the CALB-SDN of mice results from an estrogen-dependent difference in cell number with no evidence found for greater spread of cells in females. Blocking Bax-dependent cell death does not prevent sex differences in calbindin-ir cell number in the BNST or CALB-SDN but increases calbindin-ir cell number in the BNSTp of both sexes.
The sexually dimorphic nucleus of the preoptic area (SDN-POA) was discovered over 30 years ago in rats and is arguably the best-studied sex difference in the mammalian brain [1, 2]. Based on measurements in Nissl-stained sections the nucleus is several times larger in volume in male rats than in females, and similar sex differences have been described in the medial POA of other mammals, including gerbils, guinea pigs, sheep, ferrets, hyenas, monkeys, and humans [3–10].
The sex difference in SDN-POA volume in rats depends on estrogenic metabolites of testosterone and is thought to be due to differential cell death during postnatal development. Male rats have more cells in the SDN-POA than do females, beginning by the second postnatal week [11–14], and females have a higher rate of cell death between postnatal days 7 and 10 [12, 15, 16]. Treating females with testosterone or an estrogen around the time of birth reduces postnatal apoptosis in the SDN-POA [12, 14, 15, 17] and masculinizes SDN-POA volume in adulthood [1, 18, 19].
Cell death in the developing brain is crucially regulated by proteins of the B cell lymphoma 2 (Bcl-2) family . Bcl-2 itself is antiapoptotic, whereas Bcl-2-associated × protein (Bax) is a proapoptotic family member required for developmental cell death of neurons [21, 22]. Female rats have reduced levels of Bcl-2 and higher levels of Bax protein in the SDN-POA during the postnatal cell death period . Moreover, treatment with estradiol reverses the sex difference in the Bcl-2/Bax ratio , consistent with the idea that estradiol normally rescues cells in the SDN-POA of males via changes in Bcl-2 family proteins.
Mice overexpressing Bcl-2 or with a targeted deletion of the Bax gene have been used to directly examine the role of cell death in the development of neural sex differences [24, 25]. Despite its prominence in sexual differentiation research, the SDN-POA was not examined in these studies because mice do not have a discernable SDN-POA based on a Nissl stain . More recently, however, the calcium binding protein calbindin-D28 has been used as a marker of the SDN-POA.
Sickel and McCarthy  first showed that male rats have a striking cluster of calbindin-immunoreactive (ir) cells that nearly perfectly overlaps the Nissl-defined SDN-POA in adulthood; this cluster (hereafter referred to as the CALB-SDN) becomes sexually dimorphic by postnatal day 8 under the control of gonadal steroid hormones . Mice also have a calbindin cluster that is exclusive to, or larger in, males [28–31]. As in rats, this sex differences appears to be due to organizational effects of gonadal steroids: gonadectomy of male mice and treatment of females with testosterone on the day of birth reverses the sex difference in the CALB-SDN whereas hormone manipulations in adulthood are without effect .
Based on these observations, the CALB-SDN seems to be a good proxy for the SDN-POA. Some confusion exists, however, about whether the sex difference is due to a difference in cell number or in cell position. Whereas some studies find more calbindin-ir cells in this region in male rats or mice [28, 31, 32], others report no sex difference in overall cell number, suggesting instead that cells are either more densely packed within the (much smaller) CALB-SDN of females and/or that females have more cells in regions surrounding the cluster [29, 33, 34]. Part of the discrepancy may be due to the subjective nature of defining the CALB-SDN, especially in females. The exact location of cell counts is often not clearly defined and published photomicrographs suggest that the various studies may not all refer to the same cell group.
Given the importance of the SDN-POA in sexual differentiation research and the increased reliance on calbindin as a marker of this nucleus, we sought to resolve several questions: first, whether there is a sex difference in cell number and/or spread of calbindin-ir cells in the CALB-SDN and surrounding regions of mice; second, whether cell number and/or spread are affected by neonatal estradiol treatment of females; and third, whether cell death contributes to sexual differentiation of the CALB-SDN. Based on our current understanding of how the Nissl-defined SDN-POA develops in rats, we hypothesized that there is a sex difference in cell number in the CALB-SDN of mice that is due to estrogen-regulated, Bax-dependent cell death. Our findings support two of these three predictions. We found an estradiol-dependent sex difference in calbindin-ir cell number in mice, both within the CALB-SDN and in regions surrounding the cluster. Surprisingly, however, we did not find support for a role of cell death in establishment of the sex difference: Bax gene deletion does not affect the number of cells in the CALB-SDN, although it significantly increases calbindin-ir cell number in the nearby principal nucleus of the bed nucleus of the stria terminalis (BNSTp).
Animals and treatments
All procedures were approved by the Institutional Animal Care and Use Committee at the University of Massachusetts, Amherst. Mice were housed under 14:10 light/dark conditions at 22°C and provided with food and water ad libitum.
In experiment 1, C57Bl/6 pups from our breeding colony were injected on the day of birth with either 20 μg of estradiol benzoate (EB; Sigma-Aldrich, St. Louis, MO, USA) in 25 μl vehicle (90% peanut oil and 10% dimethylsulfoxide (DMSO)) or the vehicle alone (N = 6 per group). The dose of EB was based on that previously shown to masculinize BNSTp volume and cell number in mice . Mice were killed and their brains collected between postnatal days 55 to 60. Because calbindin-ir cell number in the POA of mice is not influenced by gonadal hormones in adulthood , animals remained gonadally intact. Brains were immersion fixed in 5% acrolein (Sigma-Aldrich) in 0.1 M phosphate buffer for 4 h, and then submerged in 30% sucrose. Coronal sections were cut into three series at 30 μm on a freezing microtome and stored at -20°C in cryoprotectant until use. One of the three series was thionin stained and a second was labeled for calbindin immunoreactivity, as described below.
In experiment 2, wild-type (WT; Bax +/+) and Bax knockout (KO; Bax -/-) mice were generated by pairing mice heterozygous for the Bax gene deletion on a C57Bl/6 background. Tail tissue was genotyped for Bax gene status via PCR using previously established primers . Animals were killed in adulthood (60 to 90 days of age), and their brains were fixed as above. Sections were cut into two series at 30 μm, one of which was labeled for calbindin. Four groups were compared (N = 6 per group): WT males, WT females, Bax KO males and Bax KO females. Three animals were dropped from grid counts (defined below) due to histological artifacts (folding or tearing of sections) that precluded accurate counts throughout the grid bilaterally; the final number of animals per group is indicated in each figure.
All measurements reported in this study were made on slides coded to conceal the sex and treatment of the animals.
Immunocytochemistry for calbindin
Free-floating sections were rinsed three times for 5 minutes in Tris-buffered saline (TBS; 0.05 M Tris, 0.9% NaCl, pH 7.6) then incubated in 0.05 M sodium citrate in TBS for 1 h. Sections were rinsed, placed in 0.1 M glycine in TBS for 30 minute and rinsed again before incubating in blocking solution (20% normal goat serum (NGS; Pel-Freez Biologicals, Rogers, AR, USA), 0.3% Triton-X (Labchem, Inc., Pittsburgh, PA, USA) and 1% H2O2 in TBS) for 30 minutes. Sections were then exposed to primary antibody overnight (1:5,000 mouse monoclonal anti-calbindin-D28k antiserum (C9848; clone CB-955; Sigma-Aldrich)) in 2% NGS, and 0.3% Triton-X. Sections were then rinsed and incubated in secondary antiserum (1:250 biotinylated goat anti-mouse IgG, (Vector Laboratories, Burlingame, CA, USA) in TBS with 2% NGS and 0.32% Triton-X) for 1 h, followed by three 10 minute rinses in TBS containing 0.2% Triton-X. Sections were then incubated in avidin-biotin complex in TBS (ABC Elite Kit, Vector Laboratories), rinsed, and incubated in diaminobenzidine (DAB Kit, Vector Laboratories). Sections were mounted onto slides and coverslipped with Permount (Fisher Scientific, Pittsburgh, PA, USA).
Defining the CALB-SDN
We also noted a second calbindin-ir cell group in both sexes that was more dorsal and lateral to the CALB-SDN described above (Figure 1). Depending on the plane of section, this cell group was either present in the same section as the CALB-SDN, or in the next section caudally. Sparsely distributed calbindin-ir cells were sometimes seen connecting the two cell groups. Perhaps for this reason, some previous studies may have included this more dorsal cell group in CALB-SDN analyses (as suggested by photomicrographs in refs. 29 and 30, for example). However, by comparing the sections immunoreacted for calbindin with adjacent sections stained with thionin, we concluded that the more dorsal cluster comprises the ventral-most portion of the BNSTp. These cells were therefore excluded from the CALB-SDN contour and grid counts described below (unless noted) and were included as part of the BNSTp in experiment 2.
Calbindin-ir contour and grid counts
Calbindin-ir cells in the POA were quantified in experiments 1 and 2 using two methods: contour counts and grid counts (Figure 1). A contour (defined below) was designed based on the size, shape and location of the CALB-SDN in males, allowing us to count calbindin-ir cells in a consistent location in all animals. A nine-block grid, centered on the CALB-SDN, allowed us to count cells both within the cluster and in regions of the POA surrounding the cluster.
Based on pilot work, we designed an ellipsoid contour that was superimposed on sections through the POA to count CALB-SDN cells in a consistent manner across all animals. The contour (65,000 μm2; major axis 365 μm and minor axis 220 μm) was aligned with respect to the optic chiasm and third ventricle (ventral-most part of the contour approximately 675 μm dorsal to the optic chiasm and center of the contour 275 μm lateral to the third ventricle; see Figure 1A). The size of the contour was somewhat larger than the CALB-SDN found in males so that regardless of individual differences or differences in plane of sectioning the entire cluster would be included in all animals. Because the total number of calbindin-ir cells in this region was relatively small (< 100), sampling was not required; all cells that came into focus within the section and were moderately to darkly labeled were counted directly. Counts were performed on the left and right sides of the relevant sections and the two highest counts were summed for each animal. In the majority of cases, the maximal counts turned out to be the left and right sides of a single section. In the remaining cases (presumably due to asymmetrical sectioning), the two maximal counts came from adjacent sections.
To address the issue of spread of calbindin-ir cells, we also designed a grid (total size 525 μm by 525 μm) divided into nine uniform blocks that was superimposed on the same sections used for contour counts. The grid was aligned with the edge of the third ventricle and positioned so that the large majority of cells in the CALB-SDN as defined above fell within the central block of the grid. The number of calbindin-ir cells was counted within each block of the grid.
Calbindin-ir cell counts in the BNSTp
The number of calbindin-ir cells was counted in the BNSTp of wild-type and Bax KO mice in experiment 2. Because the BNSTp is large and contains thousands of calbindin-ir cells, the direct counting of all cells was not possible. Instead, the optical fractionator function of StereoInvestigator was used for random systematic sampling and determination of total calbindin-ir cell number. A counting frame of 25 μm × 25 μm and a counting grid of 85 μm × 85 μm was used to sample regions throughout the nucleus; counts of medium to darkly labeled cells were made using standard stereological rules, as previously described .
Total cell density in the POA of wild-type and Bax KO animals
Coverslips were soaked off after the calbindin-ir cell counts were made in experiment 2, and sections were counterstained with thionin. The ellipsoidal contour was again superimposed on sections containing the CALB-SDN, as described above, and all cells within the contour (calbindin-positive plus thionin only) were counted.
One-way analysis of variance (ANOVA) was used to compare mean calbindin-ir cell number based on ellipsoid contour or grid counts (individual grid blocks and total) among the three groups in experiment 1. Two-way analyses of variance (sex-by-genotype) were used to compare calbindin-ir cell number in the CALB-SDN and BNSTp of WT and Bax KO mice in experiment 2. Fisher's least significant difference (LSD) post hoc tests were performed only following significant main effects or interactions.
Experiment 1: cell number in the CALB-SDN of males, females, and females treated with EB at birth
Contour and grid counts
We noted that in some cases a few cells of the CALB-SDN and/or of the ventral-most BNSTp extended into block 1 (that is, the dorsolateral corner of the grid). To determine whether this affected our results, we reanalyzed calbindin-ir counts, omitting all cells in both blocks 1 and 5. The pattern was the same: males and EB females had more calbindin-ir cells than control females (blocks 2 to 4 plus 6 to 9; P = 0.026; P = 0.029, respectively; data not shown). Thus, both within the CALB-SDN and in the regions immediately surrounding the CALB-SDN, males have more calbindin-ir cells than females.
Experiment 2: effects of Bax gene deletion on calbindin-ir cell number in the CALB-SDN and BNSTp
CALB-SDN and grid cell counts
Total cell density in thionin counterstained tissue
The absence of an effect of Bax deletion on cell number in the CALB-SDN was somewhat surprising and led us to test for an effect on total cell density in the region. Sections that had been immunoreacted for calbindin were therefore counterstained with thionin, the ellipsoidal contour was positioned as before, and counts of all cells within the contour were made. Calbindin-ir cells made up about 15% of the total cells within the ellipse. Two-way ANOVA on total cell number (calbindin positive plus calbindin negative) revealed a main effect of Bax genotype (F1, 18 = 5.2; P = 0.015), with more cells in Bax knockout than in wild-type mice (wild-type male: 786 ± 35, wild-type female: 791 ± 51, Bax KO male: 853 ± 32; Bax KO female: 938 ± 39). There was no significant effect of sex (F1, 18 = 1.3; P = 0.27) or sex-by-genotype interaction (F1, 18 = 1.01; P = 0.33) on this measure.
Male mice have a cluster of calbindin-ir cells in the POA that mirrors a similar cell group in the SDN-POA of rats [28–31]. Although previous studies uniformly report that the volume of this cluster is larger in males, some controversy exists about whether this is due to a sex difference in the number of calbindin-ir cells. Using a uniform contour superimposed in a consistent manner over sections of the POA in males and females, we confirm that the CALB-SDN contains about three times as many cells in male than in female mice. In addition, a single injection of EB on the day of birth completely masculinizes cell number within the CALB-SDN of females.
These findings support and extend the report of Orikasa and Sakuma  that male mice have more cells in the CALB-SDN than do females and that five daily injections of EB between postnatal days 1 and 5 masculinized cell number in females. In contrast, cell counts in the CALB-SDN were not affected by adult gonadectomy or hormone replacement . Similarly, in rats, volume of the CALB-SDN was reduced in males by gonadectomy on the day of birth and increased in females treated neonatally with either testosterone proionate or EB . This indicates that sexual differentiation of the CALB-SDN is due to organizational effects of estrogenic steroids. However, there may also be a role for androgens, at least in mice, because disruption of the androgen receptor results in an intermediate phenotype (that is, volume of the CALB-SDN is not significantly different from females or males), as does the neonatal treatment of female mice with the non-aromatizable androgen dihydrotestosterone [28, 30].
With the use of grid counts we also examined the number of calbindin-ir cells surrounding the main cluster. Males had more calbindin-ir cells not only within the CALB-SDN, but also in adjacent regions. This supports the interpretation that total calbindin-ir cell number in the POA is sexually dimorphic; we did not find evidence for an equal number of cells that are more scattered in females. A limitation of this conclusion is that our grid counts examined cells distributed in two dimensions (ventral to dorsal and medial to lateral) and only within the confines of the grid. We cannot rule out the possibility that females have more calbindin-ir cells scattered rostrocaudally from the central cluster or beyond the perimeter or our grid, although a simple visual inspection did not suggest either possibility.
The sex difference in cell number in the CALB-SDN mirrors the sex difference in total cell number in the rat SDN-POA which, as described above, is thought to be due primarily to estrogen-regulated developmental cell death [11–13, 15, 16, 19]. We therefore hypothesized that cell death was the mechanism underlying sexual differentiation of the CALB-SDN, and tested this by comparing wild-type and Bax knockout mice. Cell number is increased in many brain regions of Bax knockouts and several sex differences in the brain and behavior are eliminated in Bax-/- mice [25, 37–39]. Surprisingly, however, Bax gene deletion did not increase the number of calbindin-ir cells in the CALB-SDN or surrounding regions of either sex. Thus, our results do not support a role for cell death in regulating either total cell number or the sex difference in cell number in the CALB-SDN.
The calbindin cluster is often taken as representative of the SDN-POA as a whole. The current findings suggest that either the role of cell death in the SDN-POA should be reconsidered, or that there are different mechanisms underlying the differentiation of calbindin-ir cells and other cells in the nucleus. Several caveats should be considered, however. For example, it is possible that the sex difference in the number of calbindin-ir cells is in fact due to cell death, but it is Bax-independent cell death. This seems somewhat unlikely given the crucial role of Bax and Bcl-2 in controlling cell death throughout the developing brain and the fact that Bax deletion increased total cell density in the cluster region. In addition, apoptotic cells, as identified by activated caspase-3 immunoreactivity, are essentially eliminated in the medial preoptic area (as well as most other brain areas) of Bax knockout mice postnatally (Ahern T, Carr A and Forger NG, unpublished results), suggesting negligible Bax-independent cell death in this region. If cell death contributes to calbindin-ir cell number in the POA, it would have to be both Bax and caspase-3 independent.
A second possible limitation to the conclusion that cell death does not contribute to development of the CALB-SDN is that presumptive calbindin-ir cells may be rescued from cell death by Bax deletion, but fail to make calbindin protein. Supernumerary cells in the BNSTp and anteroventral periventricular nucleus of Bax knockout mice express proteins such as vasopressin, NeuN, the androgen receptor (AR) and kisspeptin [36, 40, 41], indicating that they differentiate normally to the extent of expressing at least some relevant markers. However, the 'extra' cells in Bax knockout mice may not always differentiate normally (see, for example, ). The inability of cells rescued by Bax deletion to specifically express calbindin seems unlikely here, however, because we found a significant increase in the number of calbindin-ir cells in the BNSTp of Bax knockout animals.
Taken together, these findings indicate that cell death is not the only mechanism underlying sex differences in cell number in the SDN-POA, at least not the sex difference in calbindin-ir cell number. Differential neurogenesis, migration, or phenotypic differentiation are other possible mechanisms. Previous studies examining the birthdate of cells comprising the SDN-POA of rats have not found evidence for a sex difference in neurogenesis [11, 34, 43, 44]. A sex difference in the migration or aggregation of cells into the nucleus is possible, especially in light of work demonstrating effects of sex and estradiol on cell migration in embryonic slice cultures of the POA [45, 46]. We note, however, that if differences in the migration or aggregation of cells account for sexual differentiation of the CALB-SDN, calbindin-ir cells in females must end up quite a distance away from the cluster seen in males (that is, beyond the boundaries of the grid used for counts here). A more likely mechanism may be the differentiation of neurochemical phenotype. That is, more cells may be induced to differentiate into calbindin-expressing cells in males, presumably in response to perinatal exposure to testosterone and its metabolites. Similarly, other sex differences in the medial POA of rats depend on the differentiation of (morphological) phenotype, for example, the morphology of astrocytes is more complex and the density of dendritic spines is greater in the POA of male than in female rats [47, 48].
In addition to the sex difference in the CALB-SDN, we also found a sex difference in the number of calbindin-ir cells in the BNSTp. The calbindin-ir cells in the BNSTp have not received much attention although they comprised the largest and most striking accumulation of calbindin-positive cells in our material. In the only other report on these cells that we are aware of, and consistent with the present findings, Büdefeld et al.  reported more calbindin immunoreactivity in the encapsulated portion of the BNSTp of male than of female mice. This sex difference was seen in animals that had been gonadectomized and treated with testosterone propionate in adulthood indicating that, similar to the sex difference in the CALB-SDN, it is independent of adult hormonal state .
Preventing cell death by Bax gene deletion increases the number of Nissl-stained cells and AR-expressing cells in the BNSTp and eliminates sex differences in total and AR cell number [25, 36]. Deleting Bax also increased the number of calbindin-ir cells in the BNSTp here. However, the increase was roughly equivalent (approximately 1,000 cells) in males and females so the sex difference was not eliminated. Thus, although cell death determines the number of potential calbindin-ir cells in both sexes, a second mechanism must be responsible for the sex difference. As for the CALB-SDN, we suggest that this second mechanism is the differentiation of neurochemical phenotype (that is, more cells become calbindin-expressing neurons in males, under the control of hormones or sex chromosomes).
The fact that Bax deletion increased calbindin-ir cell number in the BNSTp but not the CALB-SDN raises the question of what underlies this regional difference. Although calbindin has been used as a 'marker' of the SDN-POA it is also a calcium binding protein associated with neuroprotection (see, for example, [49–51]). In rats, calbindin is expressed at higher levels in males in the preoptic area/mediobasal hypothalamus generally, and the SDN-POA specifically, prior to the period of sexually dimorphic cell death [27, 52, 53]. It is therefore possible that perinatal testosterone directs more cells to become calbindin-expressing in males and that those cells with calbindin protein are relatively protected from cell death. That is, Bax gene deletion may have had no effect on the number of cells in the CALB-SDN because calbindin-expressing cells are normally spared from pruning in wild-type animals. To our knowledge, it is not known if the ontogeny of calbindin expression differs between the CALB-SDN and BNSTp, but an earlier emergence of calbindin in the CALB-SDN could be one possible explanation for why Bax deletion influences calbindin cell number differently in the two nuclei.
Male mice have more cells in the CALB-SDN and surrounding areas than do females, and these sex differences are prevented by treating females with estradiol at birth. Although the sex difference in the CALB-SDN is due to cell number, it does not appear to depend on developmental cell death. This suggests that cell death is not the only mechanism controlling sex differences in the SDN-POA and caution may be warranted in using findings from the subpopulation of cells expressing calbindin as a proxy for processes operating in the SDN-POA as a whole.
We thank Jill McCutcheon for excellent technical assistance and Lynn Bengston for help with the figures. We are also grateful to Geert de Vries, Matthew Paul, Diane Kelly, Nafissa Ismail and the two anonymous reviewers who provided helpful comments on an earlier version of this manuscript. Supported by NIH R01 MH068482 (to NGF).
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