Figure 1From: Effects of blocking developmental cell death on sexually dimorphic calbindin cell groups in the preoptic area and bed nucleus of the stria terminalis Ellipse and grid contours designed to quantify calbindin-immunoreactive (ir) cell number in and surrounding the calbindin-ir sexually dimorphic nucleus in the preoptic area (CALB-SDN). The photographs in (A) and (B) are mirror-image duplications of a single image from a male mouse. (A) For CALB-SDN counts, an ellipsoid contour (solid line) was placed as indicated. A second calbindin-ir cell cluster was found dorsolateral to the CALB-SDN. These cells were determined to be the ventral extension of the bed nucleus of the stria terminalis (BNSTp) (dotted line) and were not included in CALB-SDN counts. (B) For counts of cells within and surrounding the CALB-SDN, a nine-block grid was placed as shown and counts were made within each block of the grid. Scale bar = 400 μm.Back to article page