Fig. 6

Restraint stress has different effects on the expression of voltage-gated ion channel mRNA transcripts in the PVT of males and females. Only results from males are shown in A–C and only from females from D–M to streamline this figure, since results from females were not significant in A–C and results from males were not significant from D–M. Results are not significant A In male pPVT neurons, Kcnj6 mRNA is reduced in rats restrained for 5 days compared to non-restrained controls. B In male pPVT neurons, Kcnh3 mRNA is increased in rats restrained for 5 days compared to non-restrained controls. C In male pPVT neurons, Kcnk1 mRNA is reduced following 1, but not 5, restraints compared to non-restrained controls. Females restrained for 5 days display increased mRNA expression of D Kcnb2, E Kcnk1, F Kcnn1, and G Kcnn2 in the pPVT compared to non-restrained controls. Females restrained for 5 days display decreased mRNA expression of H Kcnmb4 and I Kcnd2 in the pPVT compared to non-restrained controls. Females restrained for 5 days display increased mRNA expression of J Scn2a1, K Scnb2, L Scn8a, and M Slc12a5 in the pPVT compared to non-restrained controls. Bars indicate mean ± SEM. Asterisks indicate Dunnett’s post-hoc differences compared to non-restrained controls following one-way ANOVA. *p < 0.01, **p < 0.001. For non-restrained males, n = 7. For all other groups, n = 8