Fig. 4

In situ cytokine analysis and CD25 levels. a Cytokine levels from infected male PBMCs: IL-2, IL-4, IL-6, IFN-γ, and TNF-α compared to uninfected PBMCs. N = 40 (21 males and 19 females). Cytokine analysis was done on the first data set using one well of the triplicate samples set up (no additional hormones) and measured using the Bio-Plex protein array or ELISA, and the data was analyzed using a two-way ANOVA and Bonferroni Correction. Error bars are SE. Statistical significance is indicated as follows: *p < 0.05. b Levels of CD25⁺ cells between male and female uninfected and infected PBMCS after 7 days of infection as measured by cytometric analysis. The total percentage given represents the total percentage of cells expressing CD25⁺. N = 5 (three males and two females). This experiment was done in triplicate and the results averaged to create each data point. Data was analyzed using MANOVA with simple contrasts. Error bars are SE; n.s. not significant