Sex differences in gut fermentation and immune parameters in rats fed an oligofructose-supplemented diet

Table 5 Immune cell phenotypes in the mesenteric lymph node (MLN) and spleen of males and female rats consuming either the control rat chow or chow supplemented with OF

Tissue	Cell phenotype^a	Male		Female		Diet^b	Sex^c	Diet × sex^d
Tissue	Cell phenotype^a	Control	OF	Control	OF	Diet^b	Sex^c	Diet × sex^d
MLN	CD3⁺	72.62 ± 0.86	72.97 ± 1.47	76.98 ± 0.22	76.94 ± 1.25	NS	0.002	NS
	CD3⁺4⁺	52.19 ± 0.69	52.59 ± 0.73	54.79 ± 0.76	52.82 ± 1.43	NS	NS	NS
	CD3⁺8⁺	19.02 ± 1.22	18.31 ± 1.02	20.29 ± 0.67	21.83 ± 1.52	NS	NS	NS
	CD45RA⁺	23.36 ± 0.78	23.00 ± 1.55	18.80 ± 0.58	18.71 ± 1.25	NS	0.001	NS
	CD3⁻161⁺	0.33 ± 0.08	0.31 ± 0.08	0.39 ± 0.09	0.28 ± 0.04	NS	NS	NS
	CD68⁺	5.94 ± 0.77	5.49 ± 0.51	8.77 ± 0.78	8.09 ± 0.46	NS	<0.001	NS
Spleen	CD3⁺	71.67 ± 1.18	69.03 ± 1.96	73.03 ± 0.94	69.23 ± 1.12	0.04	NS	NS
	CD3⁺4⁺	45.53 ± 1.51	44.11 ± 1.43	46.10 ± 0.94	42.65 ± 1.21	NS	NS	NS
	CD3⁺8⁺	25.47 ± 0.90	24.93 ± 0.53	25.45 ± 0.67	25.04 ± 0.54	NS	NS	NS
	CD45RA⁺	18.52 ± 1.06	20.51 ± 1.39	15.41 ± 1.21	18.56 ± 1.43	NS	NS	NS
	CD3⁺161⁻	1.18 ± 0.2	1.57 ± 0.24	1.80 ± 0.18	1.38 ± 0.13	NS	NS	NS
	CD68⁺	41.58 ± 1.67	40.72 ± 1.49	45.97 ± 2.41	49.15 ± 1.28	NS	0.002	NS

OF oligofructose, NS not significant
^aCell phenotypes are expressed as the percent of the CD45⁺ (leukocyte common antigen) cell population. Values indicate the percent mean ± SEM (n = 5–6) for each immune cell type in the MLN and spleen
^bEffects of diet were detected using two-way ANOVA
^cDifferences between sexes were detected using two-way ANOVA
^dSignificance of interactions was detected using two-way ANOVA

ISSN: 2042-6410