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Transcriptional effects of gene dose reduction
Biology of Sex Differences volume 5, Article number: 5 (2014)
Large-scale gene dose reductions usually lead to abnormal phenotypes or death. However, male mammals, Drosophila, and Caenorhabditis elegans have only one X chromosome and thus can be considered as monosomic for a major chromosome. Despite the deleterious effects brought about by such gene dose reduction in the case of an autosome, X chromosome monosomy in males is natural and innocuous. This is because of the nearly full transcriptional compensation for X chromosome genes in males, as opposed to no or partial transcriptional compensation for autosomal one-dose genes arising due to deletions. Buffering, the passive absorption of disturbance due to enzyme kinetics, and feedback responses triggered by expression change contribute to partial compensation. Feed-forward mechanisms, which are active responses to genes being located on the X, rather than actual gene dose are important contributors to full X chromosome compensation. In the last decade, high-throughput techniques have provided us with the tools to effectively and quantitatively measure the small-fold transcriptional effects of dose reduction. This is leading to a better understanding of compensatory mechanisms.
Gene dose reductions are universal and have been studied in a variety of eukaryotes, including mammals [1–6], birds , flies [8, 9], worms , and yeasts . It is thought that gene dose reductions are tolerated or deleterious based on how out of balance the genome is following a dose change . Although translation is the closest direct measurement of levels of protein function [13, 14], which is what should count in the genic balance hypothesis, transcription (mRNA abundance) is typically measured. The importance of gene dose can be illustrated by co-expression levels of genes encoding proteins that are part of the same multi-subunit complex . This balance requirement may extend beyond genes encoding complex members. Although dose reductions of only a few single genes are directly deleterious (haploinsufficient), the subtle effects of dose reduction for most genes are cumulative and may eventually lead to the collapse of the regulatory network. In flies, deletions reduce viability when they reduce the gene dose for >1% of the genome and cause death when they reduce the gene dose for >3% of the genome . This is not the result of rare haploinsufficient loci scattered in the genome, as large deletions are lethal even when smaller deletions subdivided from them are viable. These data indicate that the deleterious effects of most large deletions result from the collapse of regulatory networks.
Natural monosomy of the X chromosome is a remarkable exception to the rule of detrimental effects of large gene dose imbalance. The viability of X chromosome monosomies (i.e., males) is due to compensatory mechanisms evolved to solve the genetic imbalances brought by the emergence of sex chromosomes. Female mammals and Drosophila have two X chromosomes, and males have one X chromosome and one Y chromosome. Hermaphrodite Caenorhabditis elegans have two X chromosomes, and males have one X chromosome. While still somewhat controversial, it appears that all three systems cope with monosomy by a twofold upregulation of highly expressed X-linked genes in males to balance gene expression between autosomes and sex chromosomes [17, 18]. However, they have quite different strategies to balance female X chromosome expression in the face of male dosage compensation . In Drosophila, upregulation of X chromosome expression is limited to males. In mammals, one X chromosome is globally inactivated in females, while in C. elegans, both X chromosomes in hermaphrodites are repressed by half . The common feature is that X chromosome expression relative to autosomes is maintained in both sexes. X chromosome dosage compensation is often stated as a way to balance gene expression between the sexes, but it is genic balance within a sex that must be rigorously regulated.
Dosage compensation on autosomes and X chromosomes
One of the problems with thinking about X chromosome dosage compensation in males is our rudimentary understanding of the relationship between gene dose and expression when a non-sex chromosome suffers a deletion. Effective measurements of minor-fold difference in expression are needed to track the response to gene dose reduction on autosomes. We need to understand this non-specialized response to dose in order to determine if X chromosome dosage compensation is layered over a general response or if X chromosome dosage compensation replaces a generic dosage compensation system.
Cell lines and cancer cells are good models for studying the relationship between gene dose and expression, because they are selected for growth and accumulate gene dose changes that would otherwise be selected against in the organism [9, 20]. In the absence of compensation, genes with a 50% dose reduction would have 50% of the expression. However, in Drosophila, partial transcriptional compensation for dose reductions occurs in some, but not all aneuploid cell lines [9, 21]. Generally, as gene dose increases, the expression per dose decreases and vice versa. Interestingly, gene dose reduction seems to be marginally better compensated than gene dose increase. For example, if there were no compensation, the expression level of one-dose genes and three-dose genes would be 50% and 150%, respectively, of the diploid baseline; if there were full compensation, their expression levels would both be 100%. However, in the diploid D8 cell line, the expression level of one-dose genes and three-dose genes are 75% and 133%, respectively . Interestingly, there are cell line-specific differences in levels of compensation in Drosophila cell lines. The cause of this variability is unknown, but it does suggest that compensation is not a universal effect. Perhaps, the levels of compensation are determined by the selection against deleterious effects of imbalance, where higher compensation would have evolved for more deleterious dose changes in these cells. Similarly, the higher compensation for one-dose genes than that for three-dose genes suggests that dose reduction is more deleterious to cells than dose increase.
The genetic model organisms allow for careful monitoring of baseline dosage compensation responses. For example, the DrosDel Deletion Collection has a set of Drosophila melanogaster lines with mapped deletions tiling the genome in a common genetic background . Partial transcriptional compensation for one-dose genes is also observed in these DrosDel lines. Microarray analysis has found that the expression level of one-dose genes is approximately 64% of the two-dose gene expression [23, 24]. While there may be compression of expression ratios in array studies resulting in over-estimation of compensation, and while the measurement of lowly expressed genes are problematic in both array and RNA-Seq experiments, the common trend is greater expression than expected if the relationship between DNA dose and RNA was 1:1. The consistent partial compensation observed in different studies focused on different deletions may be the result of a homogeneous dose response for all one-dose genes in whole animals. This model is essentially a dose damage response, where an unknown complex mediating a fixed twofold increase in gene expression recognizes a deletion and applies a correction to gene expression. If this hypothesis is correct, we would expect a normal distribution of expression change of one-dose genes, as observed in one analysis . While no mechanism for a dose damage response is known, it may be analogous to painting of fourth (POF). POF is a compensation protein that decorates the very small (approximately 70 genes) chromosome 4 in Drosophila, which is often lost resulting in monosomy . The expression of non-ubiquitously expressed genes on the fourth chromosome has been shown to be compensated by POF in flies, and the lack of POF causes lethality, exactly as expected for a dose damage response . A competing model is that observed levels of compensation are the result of the characteristics of gene-specific dose responses. This is a gene regulation or transcriptional homeostasis model. If this hypothesis is correct, we would expect the dose response is not uniform across genes, as observed in the extended tails towards better compensation in the distribution of expression change of one-dose genes in one analysis . Moreover, if the dose response is heterogeneous, the genetic pathways including the gene with altered gene dose should influence compensation. The consequences of incomplete or absent dosage compensation are reduced expression of the one-dose genes [17, 21, 24, 26, 27] and indirect propagating effects on two-dose genes through regulatory networks [24, 27–30]. Twenty-one different lines of Drosophila, each with unique small deletions collectively covering 5% of genes, resulted in the expression change in 80% of genes in the entire genome . In other words, the effect of a dose change is not restricted to the genes with reduced copy. There is extensive propagation of dose effects into the entire gene expression network. The fact that compensation for dose change in some aneuploid cells is stronger than in adult Drosophila with deletions and that other aneuploid cell lines show very poor compensation also argues against a strictly fixed dose response. Clearly, there is much work that remains to be done on exploring the response to gene dose, but these early studies suggest a way towards developing better compensation models. We can exploit current gene expression profiling models, but we need to examine more genes to see if there are gene-specific or region-specific compensation rules, determine the nature of variable compensation responses, and map out the propagation pathways to determine if they represent coherent biological pathways.
Although there is partial compensation for gene dose reduction on the autosomes, full compensation for X chromosome dose is observed in males. To solve the genetic imbalances brought by the dose reduction of X-linked genes, dosage compensation machinery needs to recognize the X chromosome (targeting), increase expression of X-linked genes in males (upregulation), and avoid the over-expression of the X chromosome genes in females (restriction). In Drosophila, the MSL complex is a male-specific ribonucleoprotein consisting of at least five proteins male-specific lethal 1–3 (MSL1, MSL2, and MSL3), maleless (MLE), and males absent on the first (MOF) and two non-coding RNAs (RNA on the X (roX) 1 and 2). MSL1, MSL2, roX1, and roX2 are required for targeting the X chromosome . The roX1 and roX2 loci encode non-coding RNAs that are required for the full assembly of MSL complex, as without roX RNAs, MSL complex mis-localizes to centromeric heterochromatin . The roX genes are X-linked, but when they are translocated to autosomes, high-level expression of roX genes still favors recruitment of MSL complex to the X chromosome . However, low-level expression of autosomal roX genes favors local bidirectional spreading of MSL complex, suggesting that roX RNAs are incorporated into the MSL complex when they are transcribed . It is possible that X linkage is favorable for roX function. MOF, a histone acetyltransferase that modifies histone H4 at lysine 16 (H4K16), performs the upregulation function. H4K16 acetylation not only activates transcription  but also loosens chromatin structure globally  and thus is considered to de-repress X chromosome genes in males. An alternative, less well-accepted model suggests that MOF is a global activator of transcription  and that the MSL complex sequesters MOF to the X chromosome to prevent the upregulation of autosomal genes . MOF does bind to autosomal sites in both sexes , raising the possibility that male-specific MSL complexes and non-sex-specific MOF complexes exist and compete. Both the recruitment of activator and sequestration models balance X and autosome expression in both sexes. Sex-specific regulation of MSL2 is also required to restrict MSL action to males. The translation of msl2 mRNA is inhibited by Sex-lethal (SXL), which responds to X chromosome number resulting in SXL expression and dosage compensation suppression only in females . Since MSL complex does not locate to the X chromosome without MSL2, this ensures that upregulation of X chromosome genes is limited to males.
Autosomal and X chromosome dosage compensation may be mediated by one or more mechanisms, including buffering, feedback, and feed-forward that can be used to ameliorate the effect of gene dose reduction in living systems . Buffering is the passive absorption of effects caused by dose reduction. For example, gene transcription may be considered as a chemical reaction obeying mass action kinetics, where the template gene is a catalyst and mRNAs are products. The flux (expression level) through the reaction is dependent on kinetic parameters and should not follow a linear relationship (Figure 1A) . Therefore, the 50% dose reduction from two doses to one dose should reduce expression <50% and thus result in partial compensation. However, the effect of buffering can only lower the expression change caused by dose reduction, but not eliminate it. At least some genes are overexpressed as a result of decreased gene dose, which cannot be explained by buffering . Feedback is an action triggered by the expression change to restore the balance. For example, the dose reduction of gene A leads to its expression reduction, which recruits regulator B to activate gene A and thus demonstrate compensation (Figure 1B). Feedback, as error-controlled regulation, takes action when the expression changes and thus can respond to expression change caused by any disturbance. Moreover, the effect of feedback is gene specific and may result in over-, full, partial, no, or anti-compensation. Considering the heterogeneous dose response for most dose reductions , feedback is likely to play an important role in partial compensation. However, feedback might not be sufficient to cope with the large number of dose changes due to monosomy of the X chromosome. Feed-forward is another action to restore the gene expression with reduced dose. For example, the regulator B may recognize and target the one-dose gene A and suppress A's expression change before the fact (Figure 1C). Feed-forward anticipates the dose reduction before it influences expression change and thus may be crucial for full compensation; unlike in the case of feedback, the correction occurs before the expression error is generated. Outside of the well-studied MSL-mediated feed-forward system, we have a very poor understanding of the contributions these mechanisms make to X chromosome compensation. Indeed, there may even be undiscovered feed-forward mechanisms to explain the MSL-independent full X chromosome compensation in the Drosophila male germline for example [17, 18]. We do not understand how such feed-forward themes evolved, but MSL components such as MOF are widely deployed in other species as general activators of transcription, suggesting that such general regulators can be co-opted for chromosome-wide duties.
Why is X chromosome dosage compensation better than autosomal compensation?
Full compensation, as observed for the Drosophila X chromosome, is an elegant solution evolved to eliminate the deleterious effects of dose reduction and genic imbalance, and is clearly important for male viability. If dosage compensation is so valuable, then full compensation for deletions should also be favored. Why is compensation for deletions so poor? In at least some situations, gene dose is regulated in order to produce large amounts of product. For example, there is differential amplification of genes in terminally differentiated Drosophila cell, such as those encoding eggshell proteins . Full dosage compensation would undo these advantageous dose differences. This is not restricted to Drosophila. A high rate of aneuploidy, including dose reduction, appears to be part of normal hepatocyte function in the mammalian liver . Imprinting can be viewed as an epigenetic dose reduction and is widespread in many species . Finally, there are a few X chromosome genes that escape dosage compensation . Given the multiple cases of regulated dose differences, it seems likely that universal and full compensation would be selected against.
One of the main differences between a sex chromosome and autosomal monosomy is the long path to compensation in the X chromosome lineage. Deletions and autosomal monosomies are sporadic and generally have a very short duration in the gene pool of any given species, while sex chromosome evolution is a driven process that persists in related species over many millions of years. It is possible that only sex chromosomes are fully compensated because it takes a great deal of time to fully fine-tune the process. For example, in Drosophila miranda, there are three X chromosome fragments of differing age as a result of interchromosome fusions: XL (>60 million years old), XR (approximately 15 million years old), and neo-X (approximately 1 million years old). Only partial compensation is observed on the neo-X segment even though the MSL machinery that already evolved to control X chromosome compensation is co-opted to regulate the neo-X segment [44, 45]. It seems likely that new cis-acting elements are acquired step by step over time, rather than all at once .
Sexual selection might also be important for the evolution of X chromosome dosage compensation. To this point, we have used sex chromosome dosage compensation as an example of full compensation; however, this is not always the case. These exceptions might be quite informative. In birds, which have ZW sex determination (ZW females and ZZ males), only partial Z chromosome dosage compensation is detected . This partial compensation might be similar to the autosomal compensation observed in Drosophila. The ZW sex determination system dates to the dinosaurs, so we cannot explain the lack of robust compensation by the absence of fine-tuning time. We also cannot explain poor Z chromosome compensation based on the fraction of the genome linked to sex chromosomes. In Gallus gallus, the Z chromosome comprises 8% of the genome [16, 48–52], while reduced dose for 3% of the fly genome cause death . If the number of genes on the Z cannot explain poor compensation, perhaps Z had a longer time to adapt due to the peculiar nature of sex linkage, sexual selection, and mutation rate .
Sexually antagonistic genes, which are advantageous to one sex but deleterious to the other sex, will accumulate to improve the function of one sex with the cost of reducing function in the other sex. In XY systems, this results in some predictions. Sexually antagonistic genes that are advantageous to male would favor the linkage with male determinant on the proto-Y so that they can exert their advantageous functions in males (with proto-Y) while avoiding their deleterious functions in females (without proto-Y). With the accumulation of sexually antagonistic genes, the non-recombining region between proto-X and proto-Y expanded, and eventually, the recombination between sex chromosomes is suppressed in males but not in females . The absence of recombination between proto-X and proto-Y led to the gradual degeneration of the Y chromosome by accumulation of deleterious mutations in an irreversible manner (called Muller's ratchet) or through hitchhiking by favorable mutations (called selective sweeps) . First, the loss of genes on proto-Y chromosome may act as common dose reduction and be compensated through buffering and feedback in a gene-by-gene manner as the partial compensation. Later, with the degeneration of proto-Y chromosome, the number of one-dose X-linked genes increased in males . In this situation, the deleterious effect of dose reduction without full compensation is large and unavoidable, and thus an active response prior to the expression change rather than a reaction to expression change is required. In this scenario, feed-forward is strongly selected for in order to restore the expression balance between autosomes and X chromosome as well as between sexes. Instead of responding to the expression change caused by dose reduction, this system responds a priori in the sex where the X chromosome dose is expected to be one . This chromosome-wide upregulation may be achieved by a combination of partial compensation and feed-forward mechanisms [21, 36], but how these mechanisms might interact is unclear.
While in both XY and ZW systems the sex chromosomes arose from an ancestral autosome pair and genes became lost on the Y and W chromosomes due to the absence of recombination with a homolog, the two different systems might have required different dosage compensation solutions. For example, in the bird population, no W chromosomes are in males, while in Drosophila populations, no Y chromosomes are in females. Thus, unlike the autosomes, the sex chromosomes are not under selection equally in the sexes. Both XY and ZZ males should accumulate mutations faster due to the greater number of cell divisions leading to spermatogenesis relative to oogenesis. Considering the strong male-biased mutation rate, if W-linked genes degenerate slower than Y-linked genes, then the X chromosome would face a greater dose imbalance due to faster Y chromosome gene loss, thus leading to the evolution of full compensation in XY systems and a more adaptive gene regulation response in ZW systems, such as birds . In this model, the partial dosage compensation observed in birds would share many of the gene-by-gene features of autosomal dosage compensation in Drosophila. It would be interesting to determine the differences between Z chromosome and autosome dosage compensation in these species.
There is full compensation for the one-dose X-linked genes in males, while there is generally only partial compensation for one-dose genes on the autosomes of either sex. Buffering and feedback mechanisms may underlie the partial compensation, while a feed-forward mechanism is involved in the full compensation of X-linked genes in wild-type males. This hypothesis is supported by evidence from Drosophila, the most well-studied model for dosage compensation. However, there are many unsolved questions: What is the exact contribution of each mechanism to full compensation? What is the MSL-independent feed-forward mechanism? How does the MSL complex achieve twofold upregulation? etc. It is crucial to untangle compensatory mechanisms and separate potential impacting factors. For example, most systematic studies for dose response focus on autosomal genes. It is still not clear whether genes on different genomic location would have different dose response. A systematic comparison between the responses of one-dose X-linked genes and one-dose autosomal genes in homogametic sex will provide us with deeper understanding of compensatory mechanisms.
BO is the chief and ZC, KG, SK, and HS are Visiting Fellow Award recipients in the section of Developmental Genomics, Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA.
Cooper GM, Nickerson DA, Eichler EE: Mutational and selective effects on copy-number variants in the human genome. Nat Genet 2007, 39: S22-S29. 10.1038/ng2054
Schrider DR, Hahn MW: Gene copy-number polymorphism in nature. Proc Biol Sci 2010, 277: 3213–3221. 10.1098/rspb.2010.1180
Perry GH, Yang F, Marques-Bonet T, Murphy C, Fitzgerald T, Lee AS, Hyland C, Stone AC, Hurles ME, Tyler-Smith C, Eichler EE, Carter NP, Lee C, Redon R: Copy number variation and evolution in humans and chimpanzees. Genome Res 2008, 18: 1698–1710. 10.1101/gr.082016.108
Lee AS, Gutierrez-Arcelus M, Perry GH, Vallender EJ, Johnson WE, Miller GM, Korbel JO, Lee C: Analysis of copy number variation in the rhesus macaque genome identifies candidate loci for evolutionary and human disease studies. Hum Mol Genet 2008, 17: 1127–1136. 10.1093/hmg/ddn002
Liu GE, Hou Y, Zhu B, Cardone MF, Jiang L, Cellamare A, Mitra A, Alexander LJ, Coutinho LL, Dell'Aquila ME, Gasbarre LC, Lacalandra G, Li RW, Matukumalli LK, Nonneman D, Regitano LCA, Smith TPL, Song J, Sonstegard TS, Van Tassell CP, Ventura M, Eichler EE, McDaneld TG, Keele JW: Analysis of copy number variations among diverse cattle breeds. Genome Res 2010, 20: 693–703. 10.1101/gr.105403.110
Chen WK, Swartz JD, Rush LJ, Alvarez CE: Mapping DNA structural variation in dogs. Genome Res 2009, 19: 500–509.
Griffin DK, Robertson LB, Tempest HG, Vignal A, Fillon V, Crooijmans RP, Groenen MA, Deryusheva S, Gaginskaya E, Carre W, Waddington D, Talbot R, Völker M, Masabanda JS, Burt DW: Whole genome comparative studies between chicken and turkey and their implications for avian genome evolution. BMC Genomics 2008, 9: 168. 10.1186/1471-2164-9-168
Emerson JJ, Cardoso-Moreira M, Borevitz JO, Long M: Natural selection shapes genome-wide patterns of copy-number polymorphism in Drosophila melanogaster . Science 2008, 320: 1629–1631. 10.1126/science.1158078
Lee H, McManus CJ, Cho D-Y, Eaton M, Somma P, Cherbas L, Resch A, Powell S, Zhang D, May G, Zhan L, Andrews J, Celniker S, Cherbas P, Przytycka T, Gatti M, Oliver B, Graveley B, MacAlpine D: DNA copy number evolution in modENCODE cell lines. Genome Biol 2014. in press
Maydan JS, Lorch A, Edgley ML, Flibotte S, Moerman DG: Copy number variation in the genomes of twelve natural isolates of Caenorhabditis elegans . BMC Genomics 2010, 11: 62. 10.1186/1471-2164-11-62
Carreto L, Eiriz MF, Gomes AC, Pereira PM, Schuller D, Santos MA: Comparative genomics of wild type yeast strains unveils important genome diversity. BMC Genomics 2008, 9: 524. 10.1186/1471-2164-9-524
Birchler JA, Veitia RA: Gene balance hypothesis: connecting issues of dosage sensitivity across biological disciplines. Proc Natl Acad Sci U S A 2012, 109(37):14746–14753. 10.1073/pnas.1207726109
Schwanhausser B, Busse D, Li N, Dittmar G, Schuchhardt J, Wolf J, Chen W, Selbach M: Global quantification of mammalian gene expression control. Nature 2011, 473: 337–342. 10.1038/nature10098
Lu P, Vogel C, Wang R, Yao X, Marcotte EM: Absolute protein expression profiling estimates the relative contributions of transcriptional and translational regulation. Nat Biotechnol 2007, 25: 117–124. 10.1038/nbt1270
Jansen R, Greenbaum D, Gerstein M: Relating whole-genome expression data with protein-protein interactions. Genome Res 2002, 12(1):37–46. 10.1101/gr.205602
Lindsley DL, Sandler L, Baker BS, Carpenter AT, Denell RE, Hall JC, Jacobs PA, Miklos GL, Davis BK, Gethmann RC, Hardy RW, Steven AH, Miller M, Nozawa H, Parry DM, Gould-Somero M, Gould-Somero M: Segmental aneuploidy and the genetic gross structure of the Drosophila genome. Genetics 1972, 71(1):157–184.
Gupta V, Parisi M, Sturgill D, Nuttall R, Doctolero M, Dudko OK, Malley JD, Eastman PS, Oliver B: Global analysis of X-chromosome dosage compensation. J Biol 2006, 5(1):3. 10.1186/jbiol30
Deng X, Hiatt JB, Nguyen DK, Ercan S, Sturgill D, Hillier LW, Schlesinger F, Davis CA, Reinke VJ, Gingeras TR, Shendure J, Waterston RH, Oliver B, Lieb JD, Disteche CM: Evidence for compensatory upregulation of expressed X-linked genes in mammals, Caenorhabditis elegans and Drosophila melanogaster . Nat Genet 2011, 43(12):1179–1185. 10.1038/ng.948
Meller VH, Kuroda MI: Sex and the single chromosome. Adv Genet 2002, 46: 1–24.
Davoli T, Xu AW, Mengwasser KE, Sack LM, Yoon JC, Park PJ, Elledge SJ: Cumulative haploinsufficiency and triplosensitivity drive aneuploidy patterns and shape the cancer genome. Cell 2013, 155(4):948–962. 10.1016/j.cell.2013.10.011
Zhang Y, Malone JH, Powell SK, Periwal V, Spana E, Macalpine DM, Oliver B: Expression in aneuploid Drosophila S2 cells. PLoS Biol 2010, 8(2):e1000320. 10.1371/journal.pbio.1000320
Ryder E, Ashburner M, Bautista-Llacer R, Drummond J, Webster J, Johnson G, Morley T, Chan YS, Blows F, Coulson D, Reuter G, Baisch H, Apelt C, Kauk A, Rudolph T, Kube M, Klimm M, Nickel C, Szidonya J, Maróy P, Pal M, Rasmuson-Lestander A, Ekström K, Stocker H, Hugentobler C, Hafen E, Gubb D, Pflugfelder G, Dorner C, Mechler B, et al.: The DrosDel deletion collection: a Drosophila genomewide chromosomal deficiency resource. Genetics 2007, 177(1):615–629. 10.1534/genetics.107.076216
Stenberg P, Lundberg LE, Johansson AM, Ryden P, Svensson MJ, Larsson J: Buffering of segmental and chromosomal aneuploidies in Drosophila melanogaster . PLoS Genet 2009, 5(5):e1000465. 10.1371/journal.pgen.1000465
Malone JH, Cho DY, Mattiuzzo NR, Artieri CG, Jiang L, Dale RK, Smith HE, McDaniel J, Munro S, Salit M, Andrews J, Przytycka TM, Oliver B: Mediation of Drosophila autosomal dosage effects and compensation by network interactions. Genome Biol 2012, 13(4):r28. 10.1186/gb-2012-13-4-r28
Stenberg P, Larsson J: Buffering and the evolution of chromosome-wide gene regulation. Chromosoma 2011, 120(3):213–225. 10.1007/s00412-011-0319-8
Lundberg LE, Figueiredo ML, Stenberg P, Larsson J: Buffering and proteolysis are induced by segmental monosomy in Drosophila melanogaster . Nucleic Acids Res 2012, 40: 5926–5937. 10.1093/nar/gks245
Henrichsen CN, Chaignat E, Reymond A: Copy number variants, diseases and gene expression. Hum Mol Genet 2009, 18: R1-R8. 10.1093/hmg/ddp011
Dermitzakis ET, Stranger BE: Genetic variation in human gene expression. Mamm Genome 2006, 17(6):503–508. 10.1007/s00335-006-0005-y
Reymond A, Henrichsen CN, Harewood L, Merla G: Side effects of genome structural changes. Curr Opin Genet Dev 2007, 17: 381–386. 10.1016/j.gde.2007.08.009
Henikoff S: Dosage-dependent modification of position-effect variegation in Drosophila . Bioessays 1996, 18(5):401–409. 10.1002/bies.950180510
Gelbart ME, Kuroda MI: Drosophila dosage compensation: a complex voyage to the X chromosome. Development 2009, 136(9):1399–1410. 10.1242/dev.029645
Meller VH, Rattner BP: The roX genes encode redundant male-specific lethal transcripts required for targeting of the MSL complex. EMBO J 2002, 21(5):1084–1091. 10.1093/emboj/21.5.1084
Kelley RL, Lee OK, Shim YK: Transcription rate of noncoding roX1 RNA controls local spreading of the Drosophila MSL chromatin remodeling complex. Mech Dev 2008, 125: 1009–1019. 10.1016/j.mod.2008.08.003
Akhtar A, Becker PB: Activation of transcription through histone H4 acetylation by MOF, an acetyltransferase essential for dosage compensation in Drosophila . Mol Cell 2000, 5: 367–375. 10.1016/S1097-2765(00)80431-1
Robinson PJ, An W, Routh A, Martino F, Chapman L, Roeder RG, Rhodes D: 30 nm chromatin fibre decompaction requires both H4-K16 acetylation and linker histone eviction. J Mol Biol 2008, 381: 816–825. 10.1016/j.jmb.2008.04.050
Prestel M, Feller C, Becker PB: Dosage compensation and the global re-balancing of aneuploid genomes. Genome Biol 2010, 11: 216. 10.1186/gb-2010-11-8-216
Birchler JA, Pal-Bhadra M, Bhadra U: Dosage dependent gene regulation and the compensation of the X chromosome in Drosophila males. Genetica 2003, 117(2–3):179–190.
Straub T, Zabel A, Gilfillan GD, Feller C, Becker PB: Different chromatin interfaces of the Drosophila dosage compensation complex revealed by high-shear ChIP-seq. Genome Res 2013, 23(3):473–485. 10.1101/gr.146407.112
Erickson JW, Quintero JJ: Indirect effects of ploidy suggest X chromosome dose, not the X:A ratio, signals sex in Drosophila . PLoS Biol 2007, 5(12):e332. 10.1371/journal.pbio.0050332
Kacser H, Burns JA: The molecular basis of dominance. Genetics 1981, 97(3–4):639–666.
Nordman J, Orr-Weaver TL: Regulation of DNA replication during development. Development 2012, 139(3):455–464. 10.1242/dev.061838
Duncan AW, Hanlon Newell AE, Smith L, Wilson EM, Olson SB, Thayer MJ, Strom SC, Grompe M: Frequent aneuploidy among normal human hepatocytes. In Gastroenterology. Volume 142. New York: Elsevier; 2012:25–28. 10.1053/j.gastro.2011.10.029
Lee JT, Bartolomei MS: X-inactivation, imprinting, and long noncoding RNAs in health and disease. Cell 2013, 152(6):1308–1323. 10.1016/j.cell.2013.02.016
Alekseyenko AA, Ellison CE, Gorchakov AA, Zhou Q, Kaiser VB, Toda N, Walton Z, Peng S, Park PJ, Bachtrog D, Kuroda MI: Conservation and de novo acquisition of dosage compensation on newly evolved sex chromosomes in Drosophila . Genes Dev 2013, 27: 853–858. 10.1101/gad.215426.113
Marin I, Franke A, Bashaw GJ, Baker BS: The dosage compensation system of Drosophila is co-opted by newly evolved X chromosomes. Nature 1996, 383(6596):160–163. 10.1038/383160a0
Ellison CE, Bachtrog D: Dosage compensation via transposable element mediated rewiring of a regulatory network. Science 2013, 342: 846–850. 10.1126/science.1239552
Arnold AP, Itoh Y, Melamed E: A bird's-eye view of sex chromosome dosage compensation. Annu Rev Genomics Hum Genet 2008, 9: 109–127. 10.1146/annurev.genom.9.081307.164220
Hillier LW, Miller W, Birney E, Warren W, Hardison RC, Ponting CP, Bork P, Burt DW, Groenen MAM, Delany ME, Dodgson JB, Chinwalla AT, Cliften PF, Clifton SW, Delehaunty KD, Fronick C, Fulton RS, Graves TA, Kremitzki C, Layman D, Magrini V, McPherson JD, Miner TL, Minx P, Nash WE, Nhan MN, Nelson JO, Oddy LG, Pohl CS, Randall-Maher J, et al.: Sequence and comparative analysis of the chicken genome provide unique perspectives on vertebrate evolution. Nature 2004, 432: 695–716. 10.1038/nature03154
Adams MD, Celniker SE, Holt RA, Evans CA, Gocayne JD, Amanatides PG, Scherer SE, Li PW, Hoskins RA, Galle RF, George RA, Lewis SE, Richards S, Ashburner M, Henderson SN, Sutton GG, Wortman JR, Yandell MD, Zhang Q, Chen LX, Brandon RC, Rogers YH, Blazej RG, Champe M, Pfeiffer BD, Wan KH, Doyle C, Baxter EG, Helt G, Nelson CR, et al.: The genome sequence of Drosophila melanogaster . Science 2000, 287(5461):2185–2195. 10.1126/science.287.5461.2185
Stein LD, Bao Z, Blasiar D, Blumenthal T, Brent MR, Chen N, Chinwalla A, Clarke L, Clee C, Coghlan A, Coulson A, D'Eustachio P, Fitch DHA, Fulton LA, Fulton RE, Griffiths-Jones S, Harris TW, Hillier LW, Kamath R, Kuwabara PE, Mardis ER, Marra MA, Miner TL, Minx P, Mullikin JC, Plumb RW, Rogers J, Schein JE, Sohrmann M, Spieth J, et al.: The genome sequence of Caenorhabditis briggsae : a platform for comparative genomics. PLOS Biol 2003. doi:10.1371/journal.pbio.0000045
Venter JC, Adams MD, Myers EW, Li PW, Mural RJ, Sutton GG, Smith HO, Yandell M, Evans CA, Holt RA, Gocayne JD, Amanatides P, Ballew RM, Huson DH, Wortman JR, Zhang Q, Kodira CD, Zheng XH, Chen L, Skupski M, Subramanian G, Thomas PD, Zhang J, Gabor Miklos GL, Nelson C, Broder S, Clark AG, Nadeau J, McKusick VA, Zinder N, et al.: The sequence of the human genome. Science 2001, 291(5507):1304–1351. 10.1126/science.1058040
Besson V, Brault V, Duchon A, Togbe D, Bizot JC, Quesniaux VF, Ryffel B, Hérault Y: Modeling the monosomy for the telomeric part of human chromosome 21 reveals haploinsufficient genes modulating the inflammatory and airway responses. Hum Mol Genet 2007, 16(17):2040–2052. 10.1093/hmg/ddm152
Naurin S, Hansson B, Bensch S, Hasselquist D: Why does dosage compensation differ between XY and ZW taxa. Trends Genet 2010, 26: 15–20. 10.1016/j.tig.2009.11.006
Charlesworth D, Charlesworth B, Marais G: Steps in the evolution of heteromorphic sex chromosomes. Heredity (Edinb) 2005, 95: 118–128. 10.1038/sj.hdy.6800697
Charlesworth B: The evolution of chromosomal sex determination and dosage compensation. Curr Biol 1996, 6: 149–162. 10.1016/S0960-9822(02)00448-7
This work was supported by the Intramural Research Programs of the National Institutes of Health (NIDDK).
The authors declare that they have no competing interests.
BO and Z-XC conceived and wrote the manuscript. KG, HS, and SK provided intellectual input. All authors read and approved the final manuscript.
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Chen, ZX., Golovnina, K., Sultana, H. et al. Transcriptional effects of gene dose reduction. Biol Sex Differ 5, 5 (2014). https://doi.org/10.1186/2042-6410-5-5
- X chromosome
- Dosage compensation
- MSL complex