Fig. 2

Whole-brain outputs of LHb^vGlut2 neurons in both sexes. (a) Schematic of anterograde tracing strategy labeling outputs of LHb^vGlut2 neurons. (b) Representative image showing the virus injection site in the LHb (left) and clear morphology of starter cell soma (right). Scale bar (left) = 200 μm, scale bar (right) = 50 μm. (c) Heatmap showing anterograde-tracing-virus infected cell distribution in each coronal section including LHb of each sample. Each row is from one sample. n = 6 in each group. (d) Representative examples of brain-wide ipsilateral outputs from LHb vGlut2 neurons in one mouse. Scale bars, 200 μm. (e) Quantification of the normalized density of ipsilateral and contralateral downstream brain subregions from LHb vGlut2 neurons in the whole brains of both sexes. (f) Normalized density of LHb vGlut2 outputs (top) and area under the curve (AUC) (bottom) from the anterior-posterior axis covering the entire efferent brain regions. The details of abbreviations for brain subregions can be seen in the list of abbreviations