Fig. 1

Whole-brain monosynaptic ipsilateral and contralateral inputs to the LHb^vGlut2 neurons in both sexes. (a) Schematic of monosynaptic rabies virus tracing strategy. (b) Representative fluorescent micrographs of coronal brain slice containing injection site in the LHb (left) and clear morphology of starter cell soma (right). Scale bar (left) = 200 μm, scale bar (right) = 50 μm. (c) Heatmap showing starter cell distribution in each coronal section including LHb of each sample. Each row is from one sample. (d) Representative examples of brain-wide ipsilateral (Ipsi) monosynaptic inputs to LHb vGlut2 neurons in one mouse. Scale bars, 200 μm. (e) Quantification of ipsilateral and contralateral LHb vGlut2 projecting neurons expressed as a percentage of the total input cells in the whole brains of both male and female mice. (f) Input proportion (top) and area under the curve (AUC) (bottom) plots along the anterior-posterior axis covering the entire brain subregions that project to the LHb vGlut2 neurons. Data are shown as mean ± s.e.m., n = 6 in each group. The details of abbreviations for brain subregions can be seen in the list of abbreviations