Fig. 8

Experimental design (A) and schematic diagram (B) of the analysis of key regulators of GH signaling pathways in the liver of Pappa2^ko/ko and Pappa2^wt/wt mice (males and females) treated with rhGH, rhIGF1 and rhPAPP-A2 for 30 days, from PND5 to PND35. Phosphorylated forms of JAK2 (C), STAT3 (D) and STAT5 (E) are represented as mean ± S.E.M (n = 6 per experimental group). Representative immunoblots (F) are also shown. See Table S2 and Figures S4 and S7 for additional information. Two-way ANOVA and Tukey-corrected tests: */**p < 0.05/0.01 versus respective saline-treated males and females; ^#/##p < 0.05/0.01 versus respective wt/wt males and females