Fig. 2

46,XY SLCs show Sertoli marker gene expression: Whole genome transcriptional profiling of HDFa SLCs. A Principal Component Analysis of all the transcriptomes under consideration in this analysis. B Venn diagrams displaying differentially expressed genes (DEGs, fold change > 1.5, p adjusted < 0.05) between GFPC (N = 3) vs adult Sertoli cells (aSC, in brown) (N = 2) and GFPC (N = 3) vs 8TF (red) and C 6TF (blue) SLCs (N = 3). Intersection areas show co-differentially expressed genes (co-DEGs). D Heat map showing 6TF and 8TF vs. GFPC log2 fold change for indicated gonadal marker genes measured by RNAseq (n = 3). E–H Representative immuno-fluorescence images showing subcellular expression and quantification of percentage of cells showing such expression for 6TF SLCs, 8TF SLCs, and GFP controls (N = 3, n = 50–60 for each group) for (E) SOX9 (F) DMRT1 (G) AMH and (H) DHH