Fig. 1

46,XY fibroblast derived SLCs exhibit significant change in morphology over the 1-month trans-differentiation and show subcellular expression of Sertoli markers. A Mogrify-predicted transcription factors required for dermal fibroblasts to Sertoli cell trans-differentiation, # = not required to reach 95% coverage of the required genes. B Outline of the trans-differentiation strategy employed throughout this report. C Representative images of live cells belonging to the indicated groups expressing GFP across the 1-month trans-differentiation culture of 46, XY dermal fibroblasts. Morphometric analysis of cells in (C) showing shape factor (D) and area quantification (E) (N = 3, n = 50–60 for each group). *Represent p values calculated from Mann Whitney statistical tests conducted between the indicated groups