Fig. 5

Sex-specific alterations in claudin-5 expression in the brain following SPS exposure. Ventral hippocampus (vHipp) and medial prefrontal cortex (mPFC) of each animal were dissected and Western blot was performed to analyze the expression of tight junction protein claudin-5. Expression of claudin-5 in A vHipp of males and B females, C mPFC of males and D females. Representative Western blots are shown. The images have been previously published in Neurobiology of Stress [39]. The analysis was performed using one-way ANOVA, as the samples from males and females were not run simultaneously. The values are normalized to their respective controls taken as 1. Claudin-5 protein expression data in the vHipp. of females, and in the mPFC of males were non-parametrically distributed and were analyzed using Kruskal–Wallis test followed by FDR corrected multiple comparison test. The rest of the data passed the normality test and were analyzed using one-way ANOVA, followed FDR corrected multiple comparison test. Each symbol represents the value for an individual animal. vHipp (blue bars—males control n = 7, SPS-R n = 6, SPS-S n = 5; pink bars—females control n = 10, SPS-R n = 6, SPS-S n = 6);mPFC (blue bars—males control n = 7, SPS-R n = 6, SPS-S n = 5; pink bars—females control n = 8, SPS-R n = 7, SPS-S n = 6)