Fig. 6

TLR7 and TLR8 are transcriptionally non-independent in CD4⁺ T cells. Quantitative analysis of RNA FISH experiments on stimulated CD4⁺ T cells from women and XY men. Cell counts from RNA FISH experiments were processed as in Fig. 4. The forest plots display the statistic of interest for each donor and its 95% CI (dots and whiskers), together with the meta-analytical group means (diamonds) and their 95% CIs (whiskers and shaded areas). In A–C, T cells were scored regardless of the chromosome of origin of the TLR7 and TLR8 transcripts (Any X). In D–F, only cells positive for the Xa probe (Xa⁺) were counted, and the data are restricted to TLR7 and TLR8 signals observed on those Xa⁺ male or female chromosomes. A, D Percentage of T cells positive for both TLR7 and TLR8 transcripts. B, E Analysis for the obs/exp ratio of the observed number of double-positive cells to the number of cells in this category expected under the hypothesis of independent transcription of TLR7 and TLR8. C, F Analysis for Yule’s Q coefficient of association in 2 × 2 tables. The p-values in A, D test the differences between group means; in B, C, E, and F, each p-value tests the divergence of a group summary value relative to the critical value, obs/exp = 1 or Q = 0; the two-tailed p-values were derived from the group CDs in the meta-analytical summarization. Women and euploid men display deviations of opposite signs from Q = 0, signifying transcriptional non-independence of TLR7 and TLR8