Fig. 5

TLR7 and TLR8 are transcriptionally non-independent in monocytes. Quantitative analysis of RNA FISH experiments on monocytes from XX women, XY men, and XXY KS men. For each donor, individual cells were scored positive or negative for TLR7 and for TLR8 transcripts, and cell counts accordingly cross-classified as a 2 × 2 contingency table. Descriptive and analytical statistics were computed from each table (Additional file 1: S3 Data), and summarized group-wise by meta-analysis. The forest plots display the statistics of interest for each donor and its 95% CI (dots and whiskers), and the meta-analytical group means (diamonds) and their 95% CIs (whiskers and shaded areas). In A, C and E, cells were scored regardless of the chromosome of origin of the TLR7 and TLR8 transcripts (Any X). In B, D and F, only cells positive for the Xa probe (Xa⁺) were counted, and the data are restricted to TLR7 and TLR8 signals observed on Xa⁺ male or female X chromosomes. A, B Percentage of cells positive for both TLR7 and TLR8 transcripts. C, D Analysis for the obs/exp ratio of the observed number of double-positive cells to the number of cells in this category expected under the hypothesis of independent transcription of TLR7 and TLR8. E, F Analysis for Yule’s Q coefficient of association in 2 × 2 tables. The p-values in A, B test the differences between group means; in C–F, each p-value tests the divergence of a group summary value relative to the critical value, obs/exp = 1 or Q = 0; the two-tailed p-values were derived from the group CDs in the meta-analytical summarization. Women and XY men display significant deviations from Q = 0, of opposite signs, signifying transcriptional non-independence of TLR7 and TLR8