Fig. 4

Examples of TLR7 and TLR8 combined transcription profiles in euploid male and female monocytes. RNA FISH analysis of CD14⁺ monocytes from male (A–C) and female (D–F) donors. Confocal microscopy planes of cell nuclei after RNA FISH for TLR7 (green), TLR8 (red), and the Xa marker (pink). Nuclei are counterstained with DAPI (blue). A, B Detection of TLR7 or TLR8 transcripts from the single X of male cells. C, D Simultaneous transcription of TLR7 and TLR8 on the male X or on the female Xa. E, F TLR7 and TLR8 co-transcription on the female Xa with concomitant TLR7 (E) or TLR8 (F) transcriptional activity on the Xi. G Schematics of the different patterns of TLR7 and TLR8 transcription in our RNA FISH data. The percentages of nuclei (mean ± SD) in females (n = 6) and males (n = 7) for each RNA FISH profile are shown