Fig. 4

Cardiac fibrosis in male and female SHRSP rats. a Cardiac interstitial fibrosis (% positive staining). b Representative images of Masson’s trichrome staining showing collagen deposition (stained blue). Whole-heart cross sections: scale = 1 mm. Magnified images: scale = 400 µm. Values represent mean ± SEM; N = 8–11/group. ****p < 0.001 via two-way ANOVA with Sidak’s multiple comparison test