Fig. 1

Experimental timeline and menopause-induced weight gain. A Experimental timeline made using Biorender.com. Body weight was taken at the end of the study (B) and was normalized to body weight at the beginning of the study (C). Visceral adiposity was determined by isolating and weighing the visceral fat pads (D) and normalizing to body weight (E). Subcutaneous adiposity was determined by isolating and weighing the subcutaneous fat pads (F) and normalizing (G) to body weight. Data are presented as mean + SEM, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, 2-way ANOVA with Tukey’s multiple comparison test, (n = 10–13/group)