Deciphering the sex bias in housekeeping gene expression in adipose tissue: a comprehensive meta-analysis of transcriptomic studies

Table 3 Candidate suHKGs for gene expression analysis

Gene	Relative stability in male	Relative stability in female	Expression level (TPM)	Expression level (TPM) in female	Expression level (TPM) in male
PPIA	873	1589	234.597	236.1	233.6
RPL19	1129.67	137.33	1707.61	1707	1708
RPS8	194.67	178.33	952.191	944.5	957.9
RPS18	119.33	296.33	3173.82	3180	3168
UBB	228	79	252.293	249.8	254.1
UBC	267.33	706.33	432.547	396.9	447.7

Selection of housekeeping candidate genes proposed to be used as a reference to compare both sexes in gene expression analysis
PPIA and RPL19 have been experimentally validated, while RPS8, RPS18, UBB, and UBC are computationally suggested
These genes are proposed based on their sex-specific values of relative expression stability obtained from the final MetaRanking positions
Expression levels have been extracted from GTEx (given in TPM), which are high enough for detection by different technologies

ISSN: 2042-6410