Fig. 10
WCGNA-based Transcriptomic Profiling of Parent NSCs for Sex and Inter-Pregnancy Differences. WGCNA of RNA transcripts expressed in 16 cell samples, shows that parent NSCs have distinct gene networks and correlations with the trait of ‘Sex’ (female vs. male) and ‘Pregnancy’ (cells derived from three separate pregnancies) contributing to the majority of the difference between clusters. A Topological overlap matrix (TOM) plot for visualizing the weighted gene co-expression network in NSCs. B Multidimensional Scaling (MDS) plot of genes in NSCs. C Module eigengene (ME) dendrogram as the dissimilarity measurement between MEs (1st principal component), showing highest branching out of ME ‘magenta’ away from ME ‘blue’. D Heatmap of WGCNA module correlations with sample traits of ‘Pregnancy’ and ‘Sex’. The results of this analysis show that ‘Sex’ (female vs. male) is the most important determinant of module identity, with the ‘blue’, ‘lightcyan’, and ‘lightyellow’ module identifying male, and the ‘green’, ‘red’, and ‘yellow’ modules identifying female NSCs. E Bar plot of ‘Sex’ (female vs. male) trait-based module significance (average gene significance in a module) across modules in NSCs