Fig. 7
From: CPT1A in AgRP neurons is required for sex-dependent regulation of feeding and thirst
Validation of the RiboTagCpt1aKO mouse model and gene expression analysis. a Scheme of the experimental strategy to isolate AgRP neuron-specific RNAs from Cpt1aKO–RiboTag mice. b Representative images of AgRP neurons tagged with the anti-HA antibody. Scale bar, 100 µm. c Analysis by qRT-PCR of mRNA levels of Agrp, Pomc and Adh1 was performed in the input and immunoprecipitated HA samples. d, e Analysis by qRT-PCR of the mRNA levels of Agrp, Npy, Slc17a6, Slc32a1, Gad1, Dlg4 in female mice (n = 4–6) and male mice (n = 5–6). The reference gene was Gapdh for all the samples analyzed by qRT-PCR. Data are expressed as the mean ± SEM. *p < 0.05, ****p < 0.0001, using Student’s t-test