Fig. 1

Female mice are protected against diet-induced obesity. a–c Biweekly body weight change of CBA/Ca male and female mice fed with a control diet (low-fat diet, 10 kcal% fat content diets), or two high-fat diets (HFD, 45 kcal% or 60 kcal% fat content diets). All mice responded to HFDs compared to control diet. The HFD-induced weight gains appeared at an earlier time (b, c) and were much greater (a) in the males as compared with the females. Two-way ANOVA. Tukey's multiple comparisons test. ^aMain effect of age; ^bmain effect of diet; ^cinteraction; †, 10% male vs. female; ‡, 45% male vs. female; #, 60% male vs. female; @, 10% vs. 45%; &, 10% vs. 60%; %, 45% vs. 60%. d–f Fasting blood glucose measured biweekly from both female and male CBA/Ca mice. The obese male mice, but not the females, developed significant hyperglycemia. HFD feeding significantly elevated a fasting blood glucose level in male mice. Two-way ANOVA. Tukey's multiple comparisons test. a, main effect of age; ^bmain effect of diet; @, 10% vs. 45%; and, 10% vs. 60%; %, 45% vs. 60%. n = 6