Fig. 2

Airway resistance is increased in ovarectomized animals treated with estradiol in comparison to female INTACT, OVA-treated animals. After five daily airway challenges with i.n. ovalbumin, lungs were inflated then excised followed by paraffin embedding and H&E staining. A–I Histological scoring was performed by a blinded pathologist on two sections per animal, 3–4 animals per group. The data are shown as mean ± SEM. Results represent 2 independent experiments. One-way ANOVA were used to determine statistical differences followed by Tukey’s post-test to determine between groups statistical effects. Statistical significance was assigned when p-value was less than 0.05; *p < 0.05, **p < 0.01; ***p < 0.001; ****p < 0.0001