Fig. 5
From: Sex-dependent expression of neutrophil gelatinase-associated lipocalin in aortic stenosis
Effect of exogenous NGAL regulation in calcifying VICs isolated from men donors. Scatter dot plot of calcium deposits in calcifying VICs isolated from men and challenged with HP, HP + 500 ng/mL rhNGAL and HP + 100 µM GP-1 (A). Markers of inflammation (B), ECM remodelling (C) and osteogenesis (D) were assessed by ELISA in cell supernatants from siNGAL VICs under HP conditions. All comparisons have been represented as a fold change relative to Control condition. *p < 0.05; **p < 0.01; ****p < 0.0001. CCL2, chemokine (C–C motif) ligand 2 (CCL2) or monocyte chemoattractant protein 1 (MCP1). MPO myeloperoxidase, Gal-3 galectin-3, TNF-a tumour necrosis factor-a, IL interleukin, MMP metalloproteinase, TIMP tissue inhibitor of metalloproteinases, BMP bone morphogenetic protein