Fig. 4

Female RAGE deficiency protects insulin-AKT signaling in adipose tissues. A, C The treatment of insulin (100 nM) for 10 min stimulated Ser 473 phosphorylation of AKT after pretreatment with MGO (10 µM) for 16 h in eother sAT (A) or eAT (B) explants from RAGE^−/−-HFD mice. Representative immunoblots and quantification from RAGE^−/−-HFD mice as indicated. B, D The graph corresponds to the adjacent blots above and represents densitometric analyses of 3 independent experiments. *p-values indicating significance of difference are indicated in the respective bar diagrams. ns indicates no statistical significance. All group data are shown as mean ± SEM. C Ucp1, Pgc1a, Cited1, and Cox8b mRNA expression levels in the sAT of male and female RAGE^−/−-HFD mice. n = 6 per group; *p-values indicating significance of difference are indicated in the respective bar diagrams. All group data are shown as mean ± SEM