Fig. 5

Only conditioned media (CM) from female OtmiR21Δ mice increases myotube diameter. Representative images (A) and quantification of myotube diameter (B) in C2C12 cultures exposed to CM made from bones of female and male OtmiR21Δ and miR21fl/fl mice (N = 3 biological replicas/group). C Frequency distributions of the diameter of 200 to 350 myotubes measured. Each graph shows the diameter of C2C12 cells treated with CM for females or males of both genotypes. Two-way ANOVA analyses were used to determine significant differences (Additional file 2: Table S2) *p ≤ 0.05 compared to sex-matched miR21fl/fl controls, #p < 0.05 for overall male versus female comparisons, and £p < 0.05 compared to females of the same genotype