Table 1 Summary of early human placenta transcriptome studies

Tsui

https://doi.org/10.1136/jmg.2003.016881

Identify placental-specific transcripts in maternal blood

Microarray

Bulk tissue

Chorionic villi

9–12 and 38–40 wks

N

N

Mikheev

https://doi.org/10.1177/1933719108322425

Profile placenta across gestation

Microarray

Bulk tissue

Chorionic villi

6–8 and 15–16 wks

N

N

Founds

https://doi.org/10.1016/j.placenta.2008.09.015

Preeclampsia

Microarray

Bulk tissue

Chorionic villi

10–12 wks

N

N

Rull

https://doi.org/10.1016/j.placenta.2012.11.032

Recurrent miscarriage

Microarray

Bulk tissue

Placenta biopsy (chorionic villi + basal plate)

1st trimester

N

N

Sitras

https://doi.org/10.1371/journal.pone.0033294

Placental development across gestation

Microarray

Bulk tissue

Chorionic villi

9–12 wks

N

N

Pantham

https://doi.org/10.1016/j.jri.2012.03.487

Antiphospholipid antibodies

Microarray

Primary explants

Placental tissue explants

8–8.5 wks

N

N

Ghaffari-Tabrizi-Wizsy

https://doi.org/10.1159/000381766

1st trimester trophoblast vs. 3rd trimester endothelial degradome

Microarray

Primary cell cultures

Chorionic villi (1st trimester), chorionic plate (3rd trimester)

7–10 wks

N

N

Roost

https://doi.org/10.1016/j.stemcr.2015.05.002

Development from early to mid- pregnancy

RNA-seq

Bulk tissue

Amnion, chorion, chorionic villi, decidua, umbilical cord

8.2–9.6, 16–18, and 21–22 wks

Y

N

James

https://doi.org/10.1530/REP-14-0646

Characterize trophoblast subpopulations in early pregnancy

Microarray

Sorted cell (flow cytometry)

Chorionic villi

5.6–12.5 wks

N

N

Leslie

https://doi.org/10.1016/j.ajpath.2015.06.020

Preeclampsia

Microarray

Bulk tissue

Chorionic villi

9–14 wks

N

N

Lassance

https://doi.org/10.1016/j.ajog.2015.02.026

Obesity/Insulin

Microarray

Primary cell culture

Primary cytotrophoblast culture

7–12 wks

N

N

Tian

https://doi.org/10.1002/path.4694

Recurrent miscarriage

Microarray

Primary cell culture

Chorionic villi

6–12 wks

N

N

Söber

https://doi.org/10.1038/srep38439

Recurrent miscarriage

RNA-seq

Bulk tissue

Chorionic villi

6–12 wks

Y

N

Lim

https://doi.org/10.1371/journal.pone.0181155

Placental development across gestation (w/ linked methylation data)

RNA-seq

Bulk tissue

Chorionic villi

1st and early 2nd trimester

N

N

Lim

https://doi.org/10.1186/s12864-017-3993-y

Chromosomal abnormalities

Microarray

Bulk tissue

Chorionic villi

11–13 wks

N

N

Weisblum

https://doi.org/10.1128/JVI.01905-16

Zika virus infection

RNA-seq

Organoid cultures

Primary decidual and chorionic villus organoid cultures

Early- and mid-gestation

N

N

Soncin

https://doi.org/10.1242/dev.156273

Profile placenta across gestation

Microarray

Bulk tissue

Chorionic villi

4–39 weeks

N

N

Liu

https://doi.org/10.1038/s41422-018-0066-y

Characterize placental cell subpopulations

RNA-seq

Single cell w/ pre-sorting (magnetic bead)

Chorionic villi (1st trimester), basal plate (2nd trimester)

8 and 24 wks

N

N

Suryawanshi

https://doi.org/10.1126/sciadv.aau4788

Characterize cells from first-trimester maternal–fetal interface

RNA-seq

Single cell and bulk tissue

Chorionic villi and decidua

6–11 wks

Y

N

Vento-Tormo

https://doi.org/10.1038/s41586-018-0698-6

Characterize cells from first-trimester maternal–fetal interface

RNA-seq

Single cell w/ pre-sorting (flow cytometry)

Chorionic villi and decidua

6–14 wks

N

N

Turco

https://doi.org/10.1038/s41586-018-0753-3

Validate source and source-derived organoid

Microarray

Bulk tissue

Chorionic villi

6–8 and 10–12 wks

N

N

Huang

https://doi.org/10.1016/j.ebiom.2018.11.015

Recurrent miscarriage

RNA-seq

Bulk tissue

Chorionic villi

 < 20 wks

N

N

Zhao

https://doi.org/10.19723/j.issn.1671-167X.2019.01.026

IVF

Microarray

Bulk tissue

Chorionic villi

7–8 wks

N

N

Gonzalez

https://doi.org/10.1186/s13293-018-0165-y

Fetal sex differences

RNA-seq

Bulk tissue

Chorionic villi

10.5–13.5 wks

Y

Y

Lee

https://doi.org/10.1016/j.fertnstert.2018.11.005

IVF and non-IVF treatment

RNA-seq

Bulk tissue

Chorionic villi

11–13 wks

Y

N

Sun

https://doi.org/10.1210/clinem/dgaa503

Sexually dimorphic crosstalk at maternal–fetal interface

Single-cell and bulk RNA-seq

Single cell and bulk tissue

Chorionic villi and decidua

10–13 wks

Y

Y

Braun

https://doi.org/10.1007/s43032-020-00355-8

Sex differences during fetal androgen exposure

RNA-seq

Bulk tissue

Chorionic villi

11–16 wks

Y

Y

Flowers

https://doi.org/10.1093/biolre/ioab221

Sex differences in microRNA expression in first and third

trimester human placenta

Small RNA-seq

Bulk tissue

Chorionic villi and placenta collected at delivery

10.4–14.5 wks and 36.3–41.4 wks

Y

Y