Fig. 4
From: Sex-biased and parental allele-specific gene regulation by KDM6A
Diploid changes in H3K27me3 and chromatin accessibility after Kdm6a KO in BC and CB clones. A UCSC Genome browser (GRCm38/mm10) view of H3K27me3 profiles in wt and Kdm6aΔE1 confirms a general increase across the entire Hoxb locus following Kdm6a KO. wt (black); KO cells (orange). B Box plots of average ratios of H3K27me3 read coverage in BC (Kdm6aΔE1) and CB KO clones (Kdm6aΔE2.5) versus wt at promoters (± 2 kb of the TSS) of genes with similar changes between the reciprocal crosses. Downregulated and upregulated genes exhibit expected increases and decreases in H3K27me3 (***p ≤ 0.0001). C Same analysis as in (B), but for ATAC-seq average ratios of read coverage. Downregulated and upregulated genes exhibit the expected decreases and increases in chromatin accessibility (***p ≤ 0.0001; **p = 0.011). D Example profiles of H3K27me3 read coverage in wt (black) and KO cells (orange) show an increase at promoters of downregulated genes (Tnik, Has2, Ephb6) in BC and CB KO clones. (UCSC Genome browser GRCm38/mm10)