Fig. 3

Sex differences in zif268 activation of dentate adult-born cells. Photomicrographs of doublecortin-immunoreactive (ir) cells (DCX-ir: green) and zif268-ir cells (red) were taken under Zeiss Axio Scan.Z1 with a 40 × objective lens (E). Photomicrographs of IdU-ir cells (green) and zif268-ir cells (white) were taken under Olympus FV1000 confocal microscope with a 40 × objective lens (F). Photomicrographs of CldU-ir cells (red) and zif268-ir (white) cells were taken under Olympus FV1000 confocal microscope with a 40 × objective lens (G). Scale bars indicate 50 μm. Mean (± SEM) percentage of double-labeled cells in the dorsal (H) and ventral (I) dentate gyrus. Females, compared to males, had greater percentage of IdU/zif268-ir cells in the dorsal and ventral dentate gyrus, whereas there was a significant interaction effect of sex by region for the percentage of CldU/zif268-ir cells, with females having a greater percentage of CldU/zif268 in the dorsal dentate gyrus but males having a greater percentage of CldU/zif268-ir in the ventral dentate gyrus. Mean (± SEM) density of double-labeled cells in the dorsal (J) and ventral (K) dentate gyrus. There was a significant interaction effect of sex by region for the density of CldU/zif268-ir cells, with females having a greater density of CldU/zif268-ir in the dorsal dentate gyrus but males having a greater density in of CldU/zif268-ir in the ventral dentate gyrus. *Indicates p < 0.05