Fig. 1

Experimental design. A Schematic illustration of experimental timeline: subjects received one intraperitoneal injection of 5-chloro-2'-deoxyuridine on Experimental Day 1 and one intraperitoneal injection of 5-iodo-2'-deoxyuridine on Experimental Day 8. Then, subjects were tested in the contextual pattern separation task for a total of 12 days (Experimental Day 16–28), followed by an activation trial in which the rats were placed in the context previously paired with shock but received no shock (Experimental Day 29). During the contextual pattern separation task, subjects were exposed to two different contexts each day; context A a shock-paired context (context paired with foot shocks) and context A’ a neutral context (context with no foot shock). B Brain regions that were examined for functional connectivity using zif268. ACC cingulate cortex (Cg1), PrL prelimbic cortex, IL infralimbic cortex, lDS lateral dorsal striatum, mDS medial dorsal striatum, NAc nucleus accumbens core, NAs nucleus accumbens shell, LA lateral amygdala, BLA basolateral amygdala, CeA central amygdala, dDG dorsal dentate gyrus, vDG ventral dentate gyrus, dCA1 dorsal cornu ammonis 1, vCA1 ventral cornu ammonis 1, dCA3 dorsal cornu ammonis 3, vCA3 ventral cornu ammonis 3