Fig. 2

Immunolocalization of neurokinin 3 receptor (Nk3R) with androgen receptor (AR), estrogen receptor α (ERα), and estrogen receptor β (ERβ). A Immunolocalization of Nk3R in the medial prefrontal cortex (mPFC) or the dorsal hippocampus (DH). B Immunolocalization of AR in the mPFC or the DH. C Immunolocalization of ERβ in the mPFC or the DH. D Immunolocalization of ERα in the mPFC or the DH. D Representative confocal images of AR, ERβ, and ERα with Nk3R in the CG and CA1. CG cingulate cortex, IL infralimbic cortex, PrL prelimbic cortex, CA1 field CA1 of hippocampus, CA3 field CA3 of hippocampus, DG dentate gyrus. Data are mean ± SEM. Yellow pixels represent colocalization between red and green. White pixels represent the colocalization of red, green, and blue. Datasets were analyzed using two-way ANOVA. **p < 0.01, ***p < 0.001. Asterisks above a line in A and D indicate a significant main effect of sex independently of the area. Asterisks in B and C indicate significant main effect sex in discrete areas after finding a significant Sex × Area interaction. E Yellow pixels indicate colocalization between Nk3R and AR, ERβ, or ERα. White pixels indicate colocalization between DAPI, Nk3R, and AR, ERβ, or ERα. Scale bar = 25 µm