Fig. 7

The HDAC inhibitor trichostatin A treatment in the female gonads increased the expression of ovarian genes and gonadal cell proliferation. A Representative IGV browser view of Zar1, Lingo3, Scnn1g and Tfcp2l1, which are female left-specific H3K27ac-associated genes (top). Note the enriched H3K27ac depositions around the regulatory regions of the selected genes and the higher mRNA expression in the female left comparing to the female right. Quantitative PCR verification of the gene expression levels after TSA treatment (bottom). Data were mean ± SEM, n = 4, * p < 0.05 by Student’s t test. B Representative images of immunofluorescence staining of the cell proliferation marker MCM2 in different female gonads treated with TSA or DMSO. Note the TSA-treated female right gonad showed increased cell proliferation compared to the DMSO treated female right gonad. The proportions of MCM2⁺ cells were quantified from each group of gonads (n ≥ 5). Data were mean ± SEM, ** p < 0.01 by Student’s t test. Scale bar: 20 μm