Fig. 5

The overall H3K27ac distribution on Z chromosome is higher in ZZ males than in ZW females. A The average expression levels of all genes from autosome, Z and W chromosomes in different sexes. The autosome gene expression was comparable between two sexes. The general expression level of Z genes was almost two times higher in ZZ males than in ZW females. The W genes were expressed only in ZW females. B The average H3K27ac modification level across the whole Z chromosome genes is almost two times higher in males than in females. H3K27ac ChIP BAM files were used to calculate the reads coverage for autosome and sex chromosomes. The coverage calculation is done for consecutive bins of 2 kilobases. C The IGV shows that both the H3K27ac occupancy near Dmrt1 locus and the expression level of Dmrt1 mRNA is higher in males than females. Note the widespread deposition of H3K27ac around Dmrt1 locus in both sexes but much higher intensity was observed in males. D The IGV indicates that regulatory region of MHM locus is dosage compensated as shown by the higher H3K27ac signal in ZW females than in ZZ males. The non-coding RNA is only transcribed from the single Z chromosome in females but not expressed in males