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Fig. 1 | Biology of Sex Differences

Fig. 1

From: Characterisation of cardiac health in the reduced uterine perfusion pressure model and a 3D cardiac spheroid model, of preeclampsia

Fig. 1

RUPP surgery increases collagen deposition and BNP45 concentration in rat hearts. a, b Paraffin-embedded rat hearts were sectioned at 10μm thickness and stained with picrosirius red to visualise collagen I/III (red) and muscle cell cytoplasm (yellow) in sham and RUPP rats. Scalebar = 50 μm. Images taken at × 5 using an Axioscan microscope were analysed for percentage area stained red to quantify collagen deposition as an indicator of cardiac fibrosis. c Rat heart protein isolated by homogenisation with RIPA lysis buffer was quantified by indirect ELISA against known standards to quantify protein concentration of BNP45. Data points are expressed as mean percentage ± SEM; n ≥ 6, unpaired Student’s t-test; *p < 0.05, ***p < 0.001

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Biology of Sex Differences

ISSN: 2042-6410

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