Fig. 6

Neurotoxic effects of hormones in an oxidative stress environment. Alone, testosterone, 17β-estradiol, and DHT-BSA have no effect on cell viability. H₂O₂ decreased cell viability by ~ 20%. Testosterone, 17β-estradiol, and DHT-BSA further decreased cell viability in the presence of oxidative stress in N27 cells (a–c) and C6 cells (d–f). Results were determined by ANOVA followed by Fisher LSD post hoc test. Results are reported as mean + SEM. p < 0.05; *versus control, **versus H₂O₂. C, vehicle control; T, 100 nM testosterone; H, H₂O₂; HT, post-treatment T. E, 1 nM 17β-estradiol; HE, post-treatment E; D, 500 nM DHT-BSA; HD, post-treatment D