Fig. 2

Sex-specific sensitivity to high-fat diet (HFD)-induced insulin resistance with or without antibiotic pretreatment. The intraperitoneal insulin tolerance test was performed in male and female mice after 8 weeks (a and b) and 12 weeks (c and d) of HFD feeding. The AHFD group was pretreated with antibiotics for 4 weeks followed by HFD feeding. Statistical analysis of the AUC of male and female mice fed either a chow diet or HFD after 8 weeks (e) and 12 weeks (f). *p < 0.05, **p < 0.01