Fig. 2

Enumeration of PLP 139-151-specific CD4 T cells in the CNS infiltrates from EAE mice. Male and female SJL mice were immunized with PLP 139-151, and brains and spinal cords were harvested from EAE-mice that showed paralytic signs. Mononuclear cells isolated from these tissues were stained with PLP 139-151 (specific) or control (Theiler’s murine encephalomyelitis virus [TMEV] 70-86) dextramers and the dextramer⁺ CD4⁺ cells were then analyzed. Representative flow cytometric plots are shown (top panel). By establishing in situ dextramer staining technique using LSCM, PLP 139-151-specific, CD4 T cells were analyzed in the brains harvested from male and female mice (bottom panel). CD4 T cells, green; dextramers, red; merged (circles, dext⁺ CD4⁺ T cells; insets represent enlarged views of dext⁺ CD4⁺ T cells). Original magnification × 1000; bar = 20 μm. Mean ± SEM values are shown (n = 3)