Fig. 3

Sex-typing strategies based on NLGN4X/Y gene polymorphisms. a Allelic discrimination patterns based on rhAMP-SNP-detection strategies using SNP assays A (squares), B (triangles), and C (circles). Results for male donor DNA are depicted in blue, for females in magenta. Each assay was run on the identical set of male and female donor DNA samples (N = 5 each). Detection values of the respective “alleles,” i.e., NLGN4X/Y gametologues, are displayed in relative fluorescent units (RFU) calculated by the thermo cycler software. The performance result of assay C is the only one out-grouping male donors from female donors. b A total of N = 111 male and female donor DNA samples were subjected to rhAMP-analysis using the SNP_C assay. Results for male (blue) and female (magenta) donor DNA separated consistently (100%) and matched with self-reported sex identification of donors