Fig. 2From: Ischemia and reperfusion injury following cardioplegic arrest is attenuated by age and testosterone deficiency in male but not female miceTemperature regulation throughout the experiment. During 15 min of baseline (BL) perfusion the temperature of the myocardium was maintained at approximately 37 °C. Following 6 min of perfusion with hypothermic cardioplegia (CP), the temperature dropped to between 6 and 9 °C. Next, the heart was submerged in cardioplegia at room temperature and temperature was maintained between 20 and 24 °C. Finally, the heart was reperfused (RP) with Krebs-Henseleit buffer and the temperature recovered to 37 °C. The inset graph is an enlarged view of the temperature drop during cardioplegic perfusion. Values represent the mean ± SEM for 15 experimentsBack to article page