Fig. 6

Plasma Ang II and Ang-(1-7) concentrations. Plasma angiotensin (Ang)-(1-7) and Ang II concentrations measured in control diet- and high-fat diet (HFD)-fed male and female mice chronically treated with Ang-(1-7) versus saline (n = 7–12/group). a, b Females exhibited lower circulating Ang-(1-7) concentrations, with no significant effect of diet. As expected, chronic Ang-(1-7) infusion significantly increased plasma levels of this hormone, particularly in chow-fed male mice, as evidenced by interactions of drug infusion with diet and sex. c, d There were no main effects of diet or sex on plasma Ang II levels. Chronic Ang-(1-7) infusion produced reflexive increases in plasma Ang II levels, with no interactions with diet or sex. Data are mean ± SEM and were analyzed by multiple regression for main effects of sex (P_Sex), diet (P_Diet), and drug (P_Drug) and their pairwise interactions (P_Drug:Sex, P_Diet:Sex, and P_Drug:Diet)