Fig. 3

Angiotensin-(1-7) improves insulin sensitivity in obese male and female mice. a, b Raw data curves showing changes in blood glucose from baseline levels in response to insulin administration over time in control diet and high-fat diet (HFD)-induced obese male and female mice chronically treated with Ang-(1-7) or saline (n = 8-12/group). c, d Data were summarized as an area under the curve (AUC), with a more negative number representing a greater drop in glucose in response to insulin, or increased insulin sensitivity. HFD produced similar insulin resistance in males and females (less negative AUC compared to control diet). While there was no main drug effect among all groups, Ang-(1-7) significantly improved insulin sensitivity in HFD-induced obese male and female mice. Data are mean ± SEM and were analyzed by multiple regression for main effects of sex (P_Sex), diet (P_Diet), and drug (P_Drug) and their pairwise interactions (P_Drug:Sex, P_Diet:Sex, and P_Drug:Diet)