Fig. 8

Siah2 regulates expression of markers of thermogenesis in female, but not male brown adipose tissue. Gene expression of markers of a lipid metabolism, b mitochondrial function, c thermogenesis, and d inflammation were assayed in brown adipose tissue of wild-type (WT) and Siah2KO (KO) male (M) and female(F) mice after 16 weeks on the HFD using real-time qRT-PCR. e Serum leptin levels were assayed in male and female wild-type (WT) and Siah2KO (KO) at 16 weeks on the HFD. Statistical significance was determined using a two-tailed, unpaired t test, a, p < 0.05, b, p < 0.01, c, p < 0.001; Siah2KO compared to wild-type within the same sex