Fig. 4

Siah2KO affect adipocyte size and number in male, but not female gonadal and inguinal fat. Adipocyte size (area) in the LFD and HFD-fed female and male wild-type (WT) or Siah2KO (KO) mice was determined by automated cell counting of H&E stained tissue using Image J software. Adipocyte number/fat pad was estimated by converting the adipocyte area to an adipocyte volume (pL) and converting fat pad weight to a volume using the density of lipids. a Adipocyte volume (pL) and b adipocyte number/fat pad for gonadal fat. c Adipocyte volume (pL) and d adipocyte number/fat pad for inguinal fat. Statistical significance was determined using two-tailed, unpaired t test