Table 2 Proteins identified from human carotid atheroma which discriminate samples by sex, men or women, in accordance with a significant variable influence on projection (VIP ≥ 1.2) in multivariate analysis
Protein name | Accession number | Biological function | VIP | Average LFQ (E + 06) | Fold change men /women | P value | Bonferroni adjusted P value | |
|---|---|---|---|---|---|---|---|---|
Men mean (SD) | Women mean (SD) | |||||||
Cofilin-1 | P23528 | Actin organisation | 1.29 | 12.4 (12.3) | 7.9 (7.2) | 1.56 | 0.270 | – |
Transgelin-2 | P37802 | Actin organisation | 1.49 | 26.8 (28.8) | 16.8 (17.1) | 1.60 | 0.225 | – |
Coagulation factor XII | P00748 | Blood coagulation | 1.51 | 12.4 (12.3) | 7.9 (7.2) | 1.56 | < 0.001* | 0.029* |
Antithrombin-III | P01008 | Blood coagulation | 1.91 | 14.1 (5.4) | 23.3 (10.8) | 0.61 | < 0.001* | 0.018* |
Plasma protease C1 inhibitor | P05155 | Blood coagulation | 1.30 | 8.3 (6.5) | 10.5 (6.4) | 0.79 | 0.138 | – |
Heparin cofactor 2 | P05546 | Blood coagulation | 1.34 | 3.9 (3.1) | 6.2 (4.2) | 0.63 | 0.014 | 0.635 |
Protein S100-A11 | P31949 | Cell cycle | 1.26 | 7.9 (5.8) | 6.6 (5.9) | 1.20 | 0.266 | – |
Ubiquitin-60S ribosomal protein L40 | P62987 | Cell cycle | 1.86 | 5.6 (3.1) | 3.9 (2.1) | 1.42 | 0.045* | 1.950 |
Annexin A5 | P08758 | Cell death | 1.28 | 28.3 (18.5) | 23.2 (18.7) | 1.22 | 0.163 | – |
14-3-3 protein beta/alpha | P31946 | Cell death | 1.43 | 5.3 (3.5) | 4.0 (1.8) | 1.33 | 0.379 | – |
Complement factor B | P00751 | Complement activation | 1.66 | 9.7 (4.2) | 14.7 (7.9) | 0.69 | 0.025* | 1.111 |
Ig kappa chain V-II region TEW | P01617 | Complement activation | 1.59 | 5.7 (2.6) | 4.0 (1.8) | 1.43 | 0.009* | 0.408 |
CD59 glycoprotein | P13987 | Complement activation | 1.27 | 1.9 (1.2) | 2.5 (1.9) | 0.76 | 0.237 | – |
Haemoglobin subunit beta | P68871 | Haemoglobin/haptoglobin | 1.44 | 1495 (1426) | 1049 (1176) | 1.43 | 0.219 | – |
Haemoglobin subunit alpha | P69905 | Haemoglobin/haptoglobin | 1.62 | 510 (628) | 318 (415) | 1.61 | 0.164 | – |
Haptoglobin | P00738 | Haemoglobin/haptoglobin | 1.28 | 117 (151) | 161 (170) | 0.73 | 0.054 | – |
Alpha-1-antichymotrypsin | P01011 | Inflammatory response | 1.63 | 17.4 (11.1) | 29.1 (20.3) | 0.60 | 0.034* | 1.453 |
Annexin A1 | P04083 | Inflammatory response | 1.48 | 28.3 (13.7) | 22.0 (11.5) | 1.28 | 0.065 | – |
Phospholipase A2, membrane-associated | P14555 | Inflammatory response | 1.47 | 2.2 (2.0) | 1.1 (1.2) | 1.90 | 0.034* | 1.478 |
Alpha-1-acid glycoprotein | P19652 | Inflammatory response | 1.43 | 3.9 (2.0) | 7.3 (4.3) | 0.53 | 0.002* | 0.107 |
Lysozyme C | P61626 | Inflammatory response | 1.72 | 74.9 (30.0) | 10.2 (9.4) | 7.38 | 0.039* | 1.686 |
Protein S100-A6 | P06703 | Ion transport | 1.44 | 13.2 (15.7) | 8.3 (11.9) | 1.59 | 0.093 | – |
Translationally-controlled tumour protein | P13693 | Ion transport | 1.35 | 1.5 (1.0) | 1.0 (0.5) | 1.51 | 0.070 | – |
LIM and SH3 domain protein 1 | Q14847 | Ion transport | 1.23 | 4.6 (3.0) | 3.4 (3.0) | 1.37 | 0.109 | – |
Serotransferrin | P02787 | Iron homeostasis | 1.43 | 179 (61.3) | 264 (142) | 0.68 | 0.013* | 0.559 |
Haemopexin | P02790 | Iron homeostasis | 1.49 | 62.4 (33.7) | 104 (73.7) | 0.60 | 0.030* | 1.28 |
Ferritin heavy chain | P02794 | Iron homeostasis | 1.21 | 54.4 (42.5) | 42.9 (38.4) | 1.27 | 0.183 | – |
Alcohol dehydrogenase 1B | P00325 | Metabolism | 1.20 | 15.3 (16.6) | 13.0 (26.5) | 1.17 | 0.136 | – |
Alpha-enolase | P06733 | Metabolism | 1.30 | 29.3 (10.1) | 23.7 (12.0) | 1.23 | 0.062 | – |
Aminopeptidase N | P15144 | Metabolism | 1.41 | 1.5 (1.1) | 2.9 (3.7) | 0.53 | 0.053 | – |
Inter-alpha-trypsin inhibitor heavy chain H1 | P19827 | Peptidase activity (negative) | 1.62 | 11.8 (7.3) | 18.8 (17.6) | 0.63 | 0.132 | – |
Phosphatidylethanolamine-binding protein 1 | P30086 | Peptidase activity (negative) | 1.76 | 6.4 (4.4) | 3.7 (2.7) | 1.71 | 0.007* | 0.286 |
Glutathione S-transferase P | P09211 | Response to ROS | 1.58 | 5.9 (3.2) | 4.5 (3.1) | 1.31 | 0.053 | – |
Glutathione S-transferase omega-1 | P78417 | Response to ROS | 1.20 | 2.4 (2.4) | 3.2 (1.7) | 0.75 | 0.009* | 0.420 |
Peroxiredoxin-1 | Q06830 | Response to ROS | 1.42 | 12.3 (5.8) | 10.0 (6.2) | 1.23 | 0.076 | – |
SH3 domain-binding glutamic acid-rich-like protein | O75368 | Signal transduction | 1.33 | 4.8 (6.0) | 3.0 (3.0) | 1.61 | 0.178 | – |
Ribonuclease 4 | P34096 | Transcription | 1.23 | 4.1 (4.0) | 2.7 (1.5) | 1.56 | 0.100 | – |
Heterogeneous nuclear ribonucleoprotein K | P61978 | Transcription | 1.24 | 2.2 (1.2) | 1.5 (0.6) | 1.45 | 0.030* | 1.304 |
Retinol-binding protein 4 | P02753 | Transport | 1.20 | 1.8 (0.8) | 2.3 (0.9) | 0.79 | 0.051 | – |
Corticosteroid-binding globulin | P08185 | Transport | 1.41 | 0.9 (0.2) | 1.4 (0.6) | 0.68 | 0.006* | 0.275 |
Zinc-alpha-2-glycoprotein | P25311 | Transport | 1.63 | 3.1 (1.1) | 4.5 (2.3) | 0.70 | 0.041* | 1.752 |
Afamin | P43652 | Transport | 2.34 | 2.0 (0.7) | 4.4 (2.4) | 0.46 | < 0.0001* | < 0.001* |
Alpha-1B-glycoprotein | P04217 | Unknown | 1.42 | 11.0 (4.7) | 17.6 (9.1) | 0.63 | 0.002* | 0.077 |