Table 2 Primary antibodies used in this study
From: Sex- and age-related changes in GABA signaling components in the human cortex
Antigen | Host species, source, catalogue, number | Concentration | Immunogen |
|---|---|---|---|
Anti-GABAAR α1 | Rabbit, Alomone, AGA-001 | 1:1000 | Peptide QPSQDELKDNTTVFTR |
Anti-GABAAR α2 | Rabbit, Alomone, AGA-002 | 1:200 | Peptide (C)TPEPNKKPENKPA |
Anti-GABAAR α3 | Rabbit, Alomone, AGA-003 | 1:200 | Peptide QGESRRQEPGDFVKQ |
Anti-GABAAR α5 | Rabbit, Thermo Fischer, PA5-31163 | 1:200 | Recombinant fragment corresponding to amino acids 142 and 379 of human GABAAR α5 |
Anti-GABAAR γ2 | Goat, Santa Cruz, SC-131935 | 1:100 | Extracellular domain of human GABAAR γ2 |
Anti-GABAAR β3 | Mouse, Novus, NB-1-47,613 | 1:500 | Peptide corresponding to amino acids 370-433 of mouse GABAAR β3 |
Anti-GAD65 | Mouse, Millipore, MAB351 | 1:1000 | Purified rat brain glutamic acid decarboxylase |
Anti-GAD67 | Mouse, Millipore, MAB5406 | 1:200 | Recombinant GAD67 protein |
Anti-GABABR R2 | Mouse, NeuroMab, 75-124 | 1:400 | Fusion protein amino acids 861-912 of rat GABABR R2 |
Anti-GAT1 | Rabbit, Alomone, AGT-001 | 1:100 | Peptide (C)ERNMHQMTDGLDK |
Anti-GAT3 | Rabbit, Alomone, AGT-003 | 1:100 | Peptide (C)REARDKAVHERGH |
Anti-β-actin | Rabbit, Abcam, ab8227 | 1:1000 | Human β-actin amino acids 1-100 |
Anti-β-actin | Mouse, Abcam, ab6276 | 1:1000 | Peptide DDDIAALVIDNGSGK |