Fig. 3

Cyclic regulation of the 17β-estradiol is lost in DOX-treated SHRs. a, c Serum concentrations of 17β-estradiol and progesterone were measured in estrous-staged SHRs at day 0 (no treatment) (ordinary ANOVA, n = 12 per stage, *p < 0.05 according to Tukey’s multiple comparison between stages). b, d 17β-estradiol and progesterone were measured in the presence of saline, DOX and DOX + DRZ at day 6 and 13 (Two-way ANOVA, n = 3–4 per treatment per stage, *p  <0.05 according to Tukey’s multiple comparison between treatments and stages). Bars represent the mean ± SEM. e, f Correlation between the levels of serum cardiac troponin I and serum 17β-estradiol or progesterone in SHRs. The data points represent the mean ± SEM