Fig. 5
From: Axonal transport in a peripheral diabetic neuropathy model: sex-dimorphic features
Effect of short-term diabetes on Dynein. a RNA was extracted from DRG of control (Ctrl) and diabetic (streptozotocin, STZ) male (M) and female (F) rats. RT-PCR was used to analyze mRNA levels of Dynein. b Dynein protein levels analyzed by immunoblotting in DRG total extracts of Ctrl and STZ M and F rats. c Dynein protein levels analyzed by immunoblotting in isolated axoplasm of Ctrl and STZ M and F rats. Representative Western blots are presented above the graphs, with the respective loading controls (GAPDH). The densitometry of each band was analyzed, and the results are expressed as percentage of M Ctrl. The columns represent the mean ± SEM, n = 6 animals for each group. The effects of sex, diabetes, and the interaction sex by diabetes were analyzed using two-way ANOVA (significance: ###p < 0.001) followed by Bonferroni post hoc test