Fig. 1

Genetic sex determination of mice by amplification of Rbm31y and/or Rbm31x by simplex PCR. a Alignment of Rbm31y (nucleotides 997–1411) and Rbm31x (nucleotides 990–1320). Sequences highlighted in red denote mismatches and the 84 bp deleted region. Primers highlighted in green. Sex determination by simplex PCR of yolk sac lysates from samples of 129S2/SvHsd (b) and C57BL/6JOlaHsd (c) genetic backgrounds. Lane L = PCR Ranger 100 bp Ladder (GeneFlow); Lanes 1–7 = yolk sac lysates; Lane 8 = no template control. d Confirmation of methodology using ear biopsies of adult animals from 129S2/SvHsd (129) and C57BL/6JOlaHsd (BL6) backgrounds. Lane L = PCR Ranger 100 bp Ladder (GeneFlow); Lanes 1–8 = ear biopsy lysates: XY 129, XX 129, XX BL6, XX 129, XY 129, XY BL6, XY 129, XX BL6; Lane 9 = no template control. Arrows indicate 500 bp band