Fig. 2

Development of pulmonary vascular remodeling and pulmonary fibrosis in middle-aged female ApoE-deficient mice. a Immunofluorescence images showing α-smooth muscle actin stained pulmonary arterioles in young and middle-aged female ApoE-deficient mice. b Quantification of arteriolar wall thickness for vessels less than 50 μm in young (n = 3) and MA (n = 3) ApoE-difiecent mice. *p < 0.05 vs. young female (t test). Expressed as mean ± SEM. c Masson trichrome stained lung sections showing pulmonary fibrosis (blue) in young female and middle-aged female ApoE-deficient mice. d Quantification of pulmonary fibrosis in young (n = 5) and MA (n = 4) ApoE-deficient mice. *p < 0.05 vs. young female (t test). Values are expressed as mean ± SEM