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Table 1 Real-time QPCR validation of genes using hepatic samples in cohort 1

From: Bariatric surgery emphasizes biological sex differences in rodent hepatic lipid handling

Gene Name Catalog # Female Male Statistics (two-way ANOVA)
Sham VSG Sham VSG
Lipid metabolism
ACOX1 Rn01460628_m1 100 ± 7a 75 ± 5b 91 ± 3 94 ± 7 p (surgery × sex) < 0.05
CD36 Rn01442639_m1 100 ± 10a 49 ± 6b 2 ± 1c 4 ± 1c p (surgery × sex) < 0.001
CPT1A Rn00580702_m1 100 ± 9 72 ± 11 90 ± 6 79 ± 12 NS
DGAT2 Rn00584870_m1 100 ± 6a 73 ± 6b 54 ± 4b 53.1 ± 4b p (surgery × sex) < 0.05
FASN Rn00569117_m1 100 ± 12 70 ± 11 17 ± 5 14 ± 2 p (sex) < 0.001
LDLR Rn00598442_m1 100 ± 8a 64 ± 5c 29 ± 3b 31 ± 3b p (surgery × sex) < 0.01;
MGAT Rn00585985_s1 100 ± 4 82 ± 6 68 ± 5 67 ± 6 p (sex) < 0.001
PGC1 Rn00590984_m1 100 ± 12 104 ± 12 35 ± 3 68 ± 7 p (sex) < 0.001
PPARα Rn00566193_m1 100 ± 9 59 ± 8 106 ± 10 102 ± 11 p (sex) < 0.05, p (surgery) < 0.05,
PPARγ Rn00440945_m1 100 ± 13 67 ± 10 85 ± 13 75 ± 11 p(surgery) < 0.05
SREBP Rn01495769_m1 100 ± 6 68 ± 7 70 ± 10 58 ± 5 p (surgery) < 0.05, p (sex) < 0.01
Cholesterol Metabolism
ACAT2 Rn01759928_g1 100 ± 11a 66 ± 7b 20 ± 1c 21 ± 3c p (surgery × sex) < 0.05;
CYP7a1 Rn00564065_m1 100 ± 24 85 ± 11 40 ± 7 44 ± 15 p (sex) < 0.001
MTTP Rn01522970_m1 100 ± 7a 64 ± 5b 35 ± 2c 37 ± 3c p (surgery × sex) < 0.001
LRH1 Rn00572649_m1 100 ± 15a 57 ± 5b 91 ± 7a 92 ± 6a p (surgery × sex) < 0.001
Receptors
Erα Rn00433142_m1 100 ± 6a 75 ± 5b 81 ± 4b 76 ± 5b p (surgery × sex) < 0.05
FGFR1 Rn00577234_m1 100 ± 10 106 ± 6 88 ± 5 116 ± 8 p (surgery) < 0.05
FXR Rn00572658_m1 100 ± 7a 59 ± 8b 74 ± 6b 64 ± 5b p (surgery × sex) < 0.05
Gluconeogenesis
G6PC Rn01529640_g1 100 ± 4 80 ± 4 106 ± 6 93 ± 7 P(surgery) < 0.01
PCK1 Rn01529009_g1 100 ± 8 92 ± 12 158 ± 13 185 ± 16 P(sex) < 0.001
  1. Data are presented as mean ± SEM. Groups with different superscript letters are significantly different via Tukey post hoc analysis